ArticleMolecular microbiology2010
PilA localization affects extracellular polysaccharide production and fruiting body formation in Myxococcus xanthus.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Type IV pilin regulation: a transcriptional overview.Critical reviews in microbiology · 2026Review
- Article
- Tight-packing of large pilin subunits provides distinct structural and mechanical properties for theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Analysis ofFrontiers in microbiology · 2022Article
- Data-Driven Models Reveal Mutant Cell Behaviors Important for Myxobacterial Aggregation.mSystems · 2020Article
- Parallel emergence of negative epistasis across experimental lineages.Evolution; international journal of organic evolution · 2017Article
- Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis.Current microbiology · 2016Article
- Colony Expansion of Socially Motile Myxococcus xanthus Cells Is Driven by Growth, Motility, and Exopolysaccharide Production.PLoS computational biology · 2016Article
- The Matrix Reloaded: Probing the Extracellular Matrix Synchronizes Bacterial Communities.Journal of bacteriology · 2015Article
- Outside-in assembly pathway of the type IV pilus system in Myxococcus xanthus.Journal of bacteriology · 2014Article
- Killing of Escherichia coli by Myxococcus xanthus in aqueous environments requires exopolysaccharide-dependent physical contact.Microbial ecology · 2013Article
- Phosphoproteomic analysis reveals the effects of PilF phosphorylation on type IV pilus and biofilm formation in Thermus thermophilus HB27.Molecular & cellular proteomics : MCP · 2013Article
- Truncation of type IV pilin induces mucoidy in Pseudomonas aeruginosa strain PAO579.MicrobiologyOpen · 2013Article
- Type IV pilus proteins form an integrated structure extending from the cytoplasm to the outer membrane.PloS one · 2013Article
- MasABK proteins interact with proteins of the type IV pilin system to affect social motility of Myxococcus xanthus.PloS one · 2013Article
- Characterization of four type IV pilin homologues in Stigmatella aurantiaca DSM17044 by heterologous expression in Myxococcus xanthus.PloS one · 2013Article
- Type IV pilin proteins: versatile molecular modules.Microbiology and molecular biology reviews : MMBR · 2012Review
- Chemosensory signaling controls motility and subcellular polarity in Myxococcus xanthus.Current opinion in microbiology · 2012Review
- Extracellular stress and lipopolysaccharide modulate Acinetobacter baumannii surface-associated motility.Journal of microbiology (Seoul, Korea) · 2012Article
- Myxococcus xanthus developmental cell fate production: heterogeneous accumulation of developmental regulatory proteins and reexamination of the role of MazF in developmental lysis.Journal of bacteriology · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Myxococcus xanthus is a Gram-negative bacterium capable of complex developmental processes involving vegetative swarming and fruiting body formation. Social (S-) gliding motility, one of the two motility systems used by M. xanthus, requires at least two cell surface structures: type IV pili (TFP) and extracellular polysaccharides (EPS). Extended TFP that are composed of thousands of copies of PilA retract upon binding to EPS and thereby pull the cell forward. TFP also act as external sensor to regulate EPS production. In this study, we generated a random PilA mutant library and identified one derivative, SW1066, which completely failed to undergo developmental processes. Detailed characterization revealed that SW1066 produced very little EPS but wild-type amounts of PilA. These mutated PilA subunits, however, are unable to assemble into functional TFP despite their ability to localize to the membrane. By preventing the mutated PilA of SW1066 to translocate from the cytoplasm to the membrane, fruiting body formation and EPS production were restored to the levels observed in mutant strains lacking PilA. This apparent connection between PilA membrane accumulation and reduction in surface EPS implies that specific cellular PilA localization are required to maintain the EPS level necessary to sustain normal S-motility in M. xanthus.
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