Evidence map›Paper›PMID 20442932›Full record

ArticlePhotochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology2010

Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters.

Yasuko Antoku, Jun-ichi Hotta, Hideaki Mizuno, Robert M Dickson, Johan Hofkens, Tom Vosch

Open access · greenAbstract read
In one paragraph

Article in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
5.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 91 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
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  7. Review
  8. Review
  9. Tailoring silver nanodots for intracellular staining.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Yasuko AntokuDepartment of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium.
Jun-ichi Hotta
Hideaki Mizuno
Robert M Dickson
Johan Hofkens
Tom Vosch
KU Leuven · BEGeorgia Institute of Technology · US

Funding

Multifunctional fluorogenic Ag nanodots for dynamic intracellular single moleculeR01GM086195 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI DICKSON, ROBERT M · 2008 to 2011
$2.6M
NIGMS NIH HHS R01 GM086195NIGMS NIH HHS R01-GM086195
6 · The paper itself

Abstract

The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C(24):Ag(n)) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C(24):Ag(n) molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C(24):Ag(n) was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C(24):Ag(n) clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.

Indexed as

CapsulesCytosineFluorescent DyesHeLa CellsHumansMetal NanoparticlesMicroscopy, ConfocalPolymersSilverTransfectionCapsulesCytosineFluorescent DyesPolymersSilver

Identifiers

PMID20442932
PMCPMC2913586
OpenAlexW1976529111

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.