ArticleThe Plant cell2010
A soluble guanylate cyclase mediates negative signaling by ammonium on expression of nitrate reductase in Chlamydomonas.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 98 citations in OpenAlex.
- Article
- From Feasting to Fasting: The Arginine Pathway as a Metabolic Switch in Nitrogen-DeprivedCells · 2023Review
- Light-independent regulation of algal photoprotection by CONature communications · 2023Article
- Effects of nitrate and ammonium on assimilation of nitric oxide by Heterosigma akashiwo.Scientific reports · 2023Article
- Article
- Molecular Mechanisms Underlying the Acclimation ofFrontiers in plant science · 2021Article
- Identification of Partner Proteins of the AlgaeFrontiers in plant science · 2021Article
- Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis.International journal of molecular sciences · 2020Article
- Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green AlgaInternational journal of molecular sciences · 2020Article
- Article
- Characterization of tomato protein kinases embedding guanylate cyclase catalytic center motif.Scientific reports · 2020Article
- Algal photosynthesis converts nitric oxide into nitrous oxide.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A streamlined and predominantly diploid genome in the tiny marine green alga Chloropicon primus.Nature communications · 2019Article
- Article
- Role of Nitrate Reductase in NO Production in Photosynthetic Eukaryotes.Plants (Basel, Switzerland) · 2019Review
- Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.BMC biology · 2018Article
- Dual positive and negative control of Chlamydomonas PII signal transduction protein expression by nitrate/nitrite and NO via the components of nitric oxide cycle.BMC plant biology · 2018Article
- Expression of novel nitrate reductase genes in the harmful alga, Chattonella subsalsa.Scientific reports · 2018Article
- Article
- Nitric Oxide Mediates Nitrite-Sensing and Acclimation and Triggers a Remodeling of Lipids.Plant physiology · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Nitrate assimilation in plants and related organisms is a highly regulated and conserved pathway in which the enzyme nitrate reductase (NR) occupies a central position. Although some progress has been made in understanding the regulation of the protein, transcriptional regulation of the NR gene (NIA1) is poorly understood. This work describes a mechanism for the ammonium-mediated repression of NIA1. We report the characterization of a mutant defective in the repression of NIA1 and NR in response to ammonium and show that a gene (CYG56) coding for a nitric oxide (NO)-dependent guanylate cyclase (GC) was interrupted in this mutant. NO donors, cGMP analogs, a phosphodiesterase inhibitor isobutylmethylxanthine (IBMX), and a calcium ionophore (A23187) repress the expression of NIA1 in Chlamydomonas reinhardtii wild-type cells and also repress the expression of other ammonium-sensitive genes. In addition, the GC inhibitors LY83,583 (6-anilino-5,8-quinolinedione) and ODQ (1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one) release cells from ammonium repression. Intracellular NO and cGMP levels were increased in the presence of ammonium in wild-type cells. In the cyg56 mutant, NIA1 transcription was less sensitive to NO donors and A23187, but responded like the wild type to IBMX. Results presented here suggest that CYG56 participates in ammonium-mediated NIA1 repression through a pathway that involves NO, cGMP, and calcium and that similar mechanisms might be occurring in plants.
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