ArticleThe Plant cell2010
Alteration of substrate specificity: the variable N-terminal domain of tobacco Ca(2+)-dependent protein kinase is important for substrate recognition.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 97 citations in OpenAlex.
- OmicIntegrator: A Simple and Versatile Tool for Meta-Analysis.Plants (Basel, Switzerland) · 2026Article
- Research Progress onBiology · 2025Review
- Genome-wide identification, expression profile and selection analysis of the CPK gene family in Nelumbo nucifera.BMC genomics · 2025Article
- Calcium signaling-mediated transcriptional reprogramming during abiotic stress response in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- The role of CDPKs in plant development, nutrient and stress signaling.Frontiers in genetics · 2022Review
- SCARECROW-LIKE3 regulates the transcription of gibberellin-related genes by acting as a transcriptional co-repressor of GAI-ASSOCIATED FACTOR1.Plant molecular biology · 2021Article
- Plant Immune Memory in Systemic Tissue Does Not Involve Changes in Rapid Calcium Signaling.Frontiers in plant science · 2021Article
- Investigation of the Nature of CgCDPK and CgbHLH001 Interaction and the Function of bHLH Transcription Factor in Stress Tolerance inFrontiers in plant science · 2020Article
- Calcium signalling regulates the functions of the bZIP protein VIP1 in touch responses in Arabidopsis thaliana.Annals of botany · 2018Article
- Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella.Parasites & vectors · 2018Article
- Article
- Recent Advances in Substrate Identification of Protein Kinases in Plants and Their Role in Stress Management.Current genomics · 2017Review
- An Overview of Signaling Regulons During Cold Stress Tolerance in Plants.Current genomics · 2017Review
- Gibberellin Induces an Increase in Cytosolic CaPlant physiology · 2017Article
- Calcium-Dependent Protein Kinases in Phytohormone Signaling Pathways.International journal of molecular sciences · 2017Review
- Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes.Scientific reports · 2017Article
- DELLA-GAF1 Complex Is a Main Component in Gibberellin Feedback Regulation of GA20 Oxidase 2.Plant physiology · 2017Article
- The bHLH transcription factor CgbHLH001 is a potential interaction partner of CDPK in halophyte Chenopodium glaucum.Scientific reports · 2017Article
- Autophosphorylation Affects Substrate-Binding Affinity of Tobacco CaPlant physiology · 2017Article
- Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans.Plant cell reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein kinases are major signaling molecules that are involved in a variety of cellular processes. However, the molecular mechanisms whereby protein kinases discriminate specific substrates are still largely unknown. Ca(2+)-dependent protein kinases (CDPKs) play central roles in Ca(2+) signaling in plants. Previously, we found that a tobacco (Nicotiana tabacum) CDPK1 negatively regulated the transcription factor REPRESSION OF SHOOT GROWTH (RSG), which is involved in gibberellin feedback regulation. Here, we found that the variable N-terminal domain of CDPK1 is necessary for the recognition of RSG. A mutation (R10A) in the variable N-terminal domain of CDPK1 reduced both RSG binding and RSG phosphorylation while leaving kinase activity intact. Furthermore, the R10A mutation suppressed the in vivo function of CDPK1. The substitution of the variable N-terminal domain of an Arabidopsis thaliana CDPK, At CPK9, with that of Nt CDPK1 conferred RSG kinase activities. This chimeric CDPK behaved according to the identity of the variable N-terminal domain in transgenic plants. Our results open the possibility of engineering the substrate specificity of CDPK by manipulation of the variable N-terminal domain, enabling a rational rewiring of cellular signaling pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.