ArticlePlant physiology2010
Binding properties of the N-acetylglucosamine and high-mannose N-glycan PP2-A1 phloem lectin in Arabidopsis.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 99 citations in OpenAlex.
- Molecular and transcriptional regulation of plant defense responses to aphid infestation.Stress biology · 2026Review
- The SlASR4 Interaction with the Phloem Protein2 (SlPP2) regulating the tolerance to drought in tomato (Solanum lycopersicum).BMC plant biology · 2026Article
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- Comprehensive genomic and functional characterization of a phytoplasma associated with root retardation, early bolting, witches'-broom, and phyllody in daikon (Frontiers in microbiology · 2025Article
- Exploration of structural space of Cus17 lectin with glycans using molecular docking and simulation studies.Computational and structural biotechnology journal · 2025Article
- The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode.Glycobiology · 2024Article
- Molecular Interactions Between Plants and Aphids: Recent Advances and Future Perspectives.Insects · 2024Review
- PP2 gene family in Phyllostachys edulis: identification, characterization, and expression profiles.BMC genomics · 2024Article
- Arabidopsis Transcriptomics Reveals the Role of Lipoxygenase2 (AtLOX2) in Wound-Induced Responses.International journal of molecular sciences · 2024Article
- The Interplay between Enucleated Sieve Elements and Companion Cells.Plants (Basel, Switzerland) · 2023Review
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- Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis.Frontiers in plant science · 2023Article
- Alkaloid binding to opium poppy major latex proteins triggers structural modification and functional aggregation.Nature communications · 2022Article
- The Characterization of theInternational journal of molecular sciences · 2022Article
- Involvement of SUT1 and SUT2 Sugar Transporters in the Impairment of Sugar Transport and Changes in Phloem Exudate Contents in Phytoplasma-Infected Plants.International journal of molecular sciences · 2021Article
- Gene Expression Patterns for Proteins With Lectin Domains in Flax Stem Tissues Are Related to Deposition of Distinct Cell Wall Types.Frontiers in plant science · 2021Article
- Overexpression of F-Box Nictaba Promotes Defense and Anthocyanin Accumulation inFrontiers in plant science · 2021Article
- Overview of the Structure⁻Function Relationships of Mannose-Specific Lectins from Plants, Algae and Fungi.International journal of molecular sciences · 2019Review
- Identification and expression characterization of the Phloem Protein 2 (PP2) genes in ramie (Boehmeria nivea L. Gaudich).Scientific reports · 2018Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phloem Protein2 (PP2) is a component of the phloem protein bodies found in sieve elements. We describe here the lectin properties of the Arabidopsis (Arabidopsis thaliana) PP2-A1. Using a recombinant protein produced in Escherichia coli, we demonstrated binding to N-acetylglucosamine oligomers. Glycan array screening showed that PP2-A1 also bound to high-mannose N-glycans and 9-acyl-N-acetylneuraminic sialic acid. Fluorescence spectroscopy-based titration experiments revealed that PP2-A1 had two classes of binding site for N,N',N''-triacetylchitotriose, a low-affinity site and a high-affinity site, promoting the formation of protein dimers. A search for structural similarities revealed that PP2-A1 aligned with the Cbm4 and Cbm22-2 carbohydrate-binding modules, leading to the prediction of a beta-strand structure for its conserved domain. We investigated whether PP2-A1 interacted with phloem sap glycoproteins by first characterizing abundant Arabidopsis phloem sap proteins by liquid chromatography-tandem mass spectrometry. Then we demonstrated that PP2-A1 bound to several phloem sap proteins and that this binding was not completely abolished by glycosidase treatment. As many plant lectins have insecticidal activity, we also assessed the effect of PP2-A1 on weight gain and survival in aphids. Unlike other mannose-binding lectins, when added to an artificial diet, recombinant PP2-A1 had no insecticidal properties against Acyrthosiphon pisum and Myzus persicae. However, at mid-range concentrations, the protein affected weight gain in insect nymphs. These results indicate the presence in PP2-A1 of several carbohydrate-binding sites, with potentially different functions in the trafficking of endogenous proteins or in interactions with phloem-feeding insects.
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