ArticleBiophysical journal2010
Simple dark-field microscopy with nanometer spatial precision and microsecond temporal resolution.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 173 citations in OpenAlex.
- The lower γ region supports forward rotation in the latter half of the 80° substep of FProtein science : a publication of the Protein Society · 2026Article
- Mapping Nanoscale Forces and Potentials in Live Cells with Microsecond 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- High-throughput mutational analysis of FProtein science : a publication of the Protein Society · 2026Article
- Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport.iScience · 2025Article
- Functional and structural characterization of FProtein science : a publication of the Protein Society · 2025Article
- Sustainable Synthesis, Characterization, Cellular Effects of Gold Nanoparticles and Their Applications as Therapeutics in Cancer Therapy.ChemPlusChem · 2025Review
- Review
- Rational engineering of DNA-nanoparticle motor with high speed and processivity comparable to motor proteins.Nature communications · 2025Article
- Review
- Deep Learning Assisted Plasmonic Dark-Field Microscopy for Super-Resolution Label-Free Imaging.Nano letters · 2024Article
- Visualizing Single V-ATPase Rotation Using Janus Nanoparticles.Nano letters · 2024Article
- Visualizing Single V-ATPase Rotation Using Janus Nanoparticles.bioRxiv : the preprint server for biology · 2024Article
- Broadband angular spectrum differentiation using dielectric metasurfaces.Nature communications · 2024Article
- Design of allosteric sites into rotary motor VNature chemistry · 2023Article
- Rotary properties of hybrid FiScience · 2023Article
- Electrical Actuation of DNA-Based Nanomechanical Systems.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Angle-dependent rotation velocity consistent with ADP release in bacterial FFrontiers in molecular biosciences · 2023Article
- Motor generated torque drives coupled yawing and orbital rotations of kinesin coated gold nanorods.Communications biology · 2022Article
- Fiber-based 3D nano-printed holography with individually phase-engineered remote points.Scientific reports · 2022Article
- Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles.International journal of nanomedicine · 2022Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular motors such as kinesin, myosin, and F(1)-ATPase are responsible for many important cellular processes. These motor proteins exhibit nanometer-scale, stepwise movements on micro- to millisecond timescales. So far, methods developed to measure these small and fast movements with high spatial and temporal resolution require relatively complicated experimental systems. Here, we describe a simple dark-field imaging system that employs objective-type evanescent illumination to selectively illuminate a thin layer on the coverslip and thus yield images with high signal/noise ratios. Only by substituting the dichroic mirror in conventional objective-type total internal reflection fluorescence microscope with a perforated mirror, were nanometer spatial precision and microsecond temporal resolution simultaneously achieved. This system was applied to the study of the rotary mechanism of F(1)-ATPase. The fluctuation of a gold nanoparticle attached to the gamma-subunit during catalytic dwell and the stepping motion during torque generation were successfully visualized with 9.1-mus temporal resolution. Because of the simple optics, this system will be applicable to various biophysical studies requiring high spatial and temporal resolution in vitro and also in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.