ArticleBiophysical journal2010
Urea facilitates the translocation of single-stranded DNA and RNA through the alpha-hemolysin nanopore.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Through the holes: the biotechnological potential of actinoporins (and other PFPs).Biophysical reviews · 2026Review
- Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force.Nature biotechnology · 2024Article
- Applications and potentials of nanopore sequencing in the (epi)genome and (epi)transcriptome era.Innovation (Cambridge (Mass.)) · 2021Review
- Stable polymer bilayers for protein channel recordings at high guanidinium chloride concentrations.Biophysical journal · 2021Article
- Fabrication and Applications of Solid-State Nanopores.Sensors (Basel, Switzerland) · 2019Review
- Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors.Nature communications · 2018Article
- Preferential Binding of Urea to Single-Stranded DNA Structures: A Molecular Dynamics Study.Biophysical journal · 2018Article
- Mimicking Ribosomal Unfolding of RNA Pseudoknot in a Protein Channel.Journal of the American Chemical Society · 2015Article
- Engineering a nanopore with co-chaperonin function.Science advances · 2015Article
- Differentiation of G:C vs A:T and G:C vs G:mC Base Pairs in the Latch Zone of α-Hemolysin.ACS nano · 2015Article
- Dynamics and Energy Contributions for Transport of Unfolded Pertactin through a Protein Nanopore.ACS nano · 2015Article
- Single-molecule investigation of G-quadruplex folds of the human telomere sequence in a protein nanocavity.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Computational studies of DNA sequencing with solid-state nanopores: key issues and future prospects.Frontiers in chemistry · 2014Review
- Tuning the size and properties of ClyA nanopores assisted by directed evolution.Journal of the American Chemical Society · 2013Article
- Translocating kilobase RNA through the Staphylococcal α-hemolysin nanopore.Nano letters · 2013Article
- Electrical Current Signatures of DNA Base Modifications in Single Molecules Immobilized in the α-Hemolysin Ion Channel.Israel journal of chemistry · 2013Article
- Multistep protein unfolding during nanopore translocation.Nature nanotechnology · 2013Article
- Solid-State and Biological Nanopore for Real-Time Sensing of Single Chemical and Sequencing of DNA.Nano today · 2013Article
- Modulation of the current signatures of DNA abasic site adducts in the α-hemolysin ion channel.Chemical communications (Cambridge, England) · 2012Article
- Crown ether-electrolyte interactions permit nanopore detection of individual DNA abasic sites in single molecules.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The staphylococcal alpha-hemolysin (alphaHL) protein nanopore is under investigation as a fast, cheap detector for nucleic acid analysis and sequencing. Although discrimination of all four bases of DNA by the alphaHL pore has been demonstrated, analysis of single-stranded DNAs and RNAs containing secondary structure mediated by basepairing is prevented because these nucleic acids cannot be translocated through the pore. Here, we show that a structured 95-nucleotide single-stranded DNA and its RNA equivalent are translocated through the alphaHL pore in the presence of 4 M urea, a concentration that denatures the secondary structure of the polynucleotides. The alphaHL pore is functional even in 7 M urea, and therefore it is easily stable enough for analyses of challenging DNA and RNA species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.