ArticleBMC developmental biology2010
Knockout of the folate transporter folt-1 causes germline and somatic defects in C. elegans.
Article in BMC developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Enhanced branched-chain amino acid metabolism improves age-related reproduction in C. elegans.Nature metabolism · 2024Article
- Folate receptor overexpression induces toxicity in a diet-dependent manner in C. elegans.Scientific reports · 2024Article
- SAMS-1 is required for the normal defecation motor program inmicroPublication biology · 2024Article
- Riboflavin depletion promotes longevity and metabolic hormesis in Caenorhabditis elegans.Aging cell · 2022Article
- High-dose folic acid supplementation results in significant accumulation of unmetabolized homocysteine, leading to severe oxidative stress in Caenorhabditis elegans.Redox biology · 2020Article
- Bacteria increase host micronutrient availability: mechanisms revealed by studies in C. elegans.Genes & nutrition · 2020Review
- Biology of theGenetics · 2019Review
- Developmental Control of the Cell Cycle: Insights fromGenetics · 2019Review
- The uridylyltransferase GlnD and tRNA modification GTPase MnmE allosterically controlThe Journal of biological chemistry · 2018Article
- Insulin Signaling Regulates Oocyte Quality Maintenance with Age via Cathepsin B Activity.Current biology : CB · 2018Article
- Effect of folate deficiency on promoter methylation and gene expression of Esr1, Cav1, and Elavl1, and its influence on spermatogenesis.Oncotarget · 2017Article
- The Adverse Effects of Triptolide on the Reproductive System of Caenorhabditis elegans: Oogenesis Impairment and Decreased Oocyte Quality.International journal of molecular sciences · 2017Article
- Bacterial Folates Provide an Exogenous Signal for C. elegans Germline Stem Cell Proliferation.Developmental cell · 2016Article
- Mating-Induced Transcriptome Changes in the Reproductive Tract of Female Aedes aegypti.PLoS neglected tropical diseases · 2016Article
- Effects of Microcystin-LR Exposure on Spermiogenesis in Nematode Caenorhabditis elegans.International journal of molecular sciences · 2015Article
- Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation.Journal of biosciences · 2015Article
- Worms, bacteria, and micronutrients: an elegant model of our diet.Trends in genetics : TIG · 2014Review
- Rec-8 dimorphism affects longevity, stress resistance and X-chromosome nondisjunction in C. elegans, and replicative lifespan in S. cerevisiae.Frontiers in genetics · 2014Article
- Identification and functional characterization of the Caenorhabditis elegans riboflavin transporters rft-1 and rft-2.PloS one · 2013Article
- Disruption of the folate pathway in zebrafish causes developmental defects.BMC developmental biology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThe C. elegans gene folt-1 is an ortholog of the human reduced folate carrier gene. The FOLT-1 protein has been shown to transport folate and to be involved in uptake of exogenous folate by worms. A knockout mutation of the gene, folt-1(ok1460), was shown to cause sterility, and here we investigate the source of the sterility and the effect of the folt-1 knockout on somatic function.
resultsOur results show that folt-1(ok1460) knockout hermaphrodites have a substantially reduced germline, generate a small number of functional sperm, and only rarely produce a functional oocyte. We found no evidence of increased apoptosis in the germline of folt-1 knockout mutants, suggesting that germline proliferation is defective. While folt-1 knockout males are fertile, their rate of spermatogenesis was severely diminished, and the males were very poor maters. The mating defect is likely due to compromised metabolism and/or other somatic functions, as folt-1 knockout hermaphrodites displayed a shortened lifespan and elongated defecation intervals.
conclusionsThe FOLT-1 protein function affects both the soma and the germline. folt-1(ok1460) hermaphrodites suffer severely diminished lifespan and germline defects that result in sterility. Germline defects associated with folate deficiency appear widespread in animals, being found in humans, mice, fruit flies, and here, nematodes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.