ArticleBMC medical genomics2010
Genomics and proteomics approaches to the study of cancer-stroma interactions.
Article in BMC medical genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 80 citations in OpenAlex.
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- Assessing the Correlation Between Langerhans Cells Population and Prognosis of Tongue Squamous Cell Carcinoma.Clinical and experimental dental research · 2025Article
- A review of standardized high-throughput cardiovascular phenotyping with a link to metabolism in mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2023Review
- LTA4H extensively associates with mRNAs and lncRNAs indicative of its novel regulatory targets.PeerJ · 2023Article
- Article
- Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis.Journal of translational medicine · 2021Article
- Prolonged cetuximab treatment promotes p27Scientific reports · 2021Article
- Cancer associated fibroblasts are distinguishable from peri-tumor fibroblasts by biological characteristics in TSCC.Oncology letters · 2019Article
- Annexin A1 peptide and endothelial cell-conditioned medium modulate cervical tumorigenesis.FEBS open bio · 2019Article
- miR-376c promotes carcinogenesis and serves as a plasma marker for gastric carcinoma.PloS one · 2017Article
- Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma.BMC complementary and alternative medicine · 2016Article
- Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines.Disease markers · 2016Article
- Changes in the gene expression of co-cultured human fibroblast cells and osteosarcoma cells: the role of microenvironment.Oncotarget · 2015Article
- Cancer-associated fibroblasts provide a suitable microenvironment for tumor development and progression in oral tongue squamous cancer.Journal of translational medicine · 2015Article
- Inflammation and cancer: role of annexin A1 and FPR2/ALX in proliferation and metastasis in human laryngeal squamous cell carcinoma.PloS one · 2014Article
- Stromal-epithelial crosstalk provides a suitable microenvironment for the progression of ovarian cancer cells in vitro.Cancer investigation · 2013Article
- Cancer-associated fibroblasts and their putative role in potentiating the initiation and development of epithelial ovarian cancer.Neoplasia (New York, N.Y.) · 2011Review
- Proteomic profiling of mucosal and submucosal colonic tissues yields protein signatures that differentiate the inflammatory colitides.Inflammatory bowel diseases · 2011Article
Corrections and comments
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Authors and funding
89 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression.
methodsThe study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells.
resultsWe observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR.
conclusionsA significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.