ArticleJournal of the American Chemical Society2010
Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics.Nature chemistry · 2024Review
- Investigating Daptomycin-Membrane Interactions Using Native MS and Fast Photochemical Oxidation of Peptides in Nanodiscs.Analytical chemistry · 2023Article
- Certain, but Not All, Tetraether Lipids from the Thermoacidophilic ArchaeonInternational journal of molecular sciences · 2021Article
- Polarization Induced Electro-Functionalization of Pore Walls: A Contactless Technology.Biosensors · 2019Review
- Nanodomain Formation in Planar Supported Lipid Bilayers Composed of Fluid and Polymerized Dienoyl Lipids.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Building membrane nanopores.Nature nanotechnology · 2017Review
- Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol.Analytical chemistry · 2017Article
- Photopolymerization of Dienoyl Lipids Creates Planar Supported Poly(lipid) Membranes with Retained Fluidity.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Decoding long nanopore sequencing reads of natural DNA.Nature biotechnology · 2014Article
- Decreased aperture surface energy enhances electrical, mechanical, and temporal stability of suspended lipid membranes.ACS applied materials & interfaces · 2013Article
- Photolithographic fabrication of microapertures with well-defined, three-dimensional geometries for suspended lipid membrane studies.Analytical chemistry · 2013Article
- Synthesis of a Diverse Library of N,N-Dimethylamino Containing Monomers Appropriate as Lipid Head Groups.Tetrahedron letters · 2011Article
- Polymerized planar suspended lipid bilayers for single ion channel recordings: comparison of several dienoyl lipids.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Methacrylate Polymer Scaffolding Enhances the Stability of Suspended Lipid Bilayers for Ion Channel Recordings and Biosensor Development.ACS biomaterials science & engineeringArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Suspended planar lipid membranes (or black lipid membranes (BLMs)) are widely used for studying reconstituted ion channels, although they lack the chemical and mechanical stability needed for incorporation into high-throughput biosensors and biochips. Lipid polymerization enhances BLM stability but is incompatible with ion channel function when membrane fluidity is required. Here, we demonstrate the preparation of a highly stable BLM that retains significant fluidity by using a mixture of polymerizable and nonpolymerizable phospholipids. Alamethicin, a voltage-gated peptide channel for which membrane fluidity is required for activity, was reconstituted into mixed BLMs prepared using bis-dienoyl phosphatidylcholine (bis-DenPC) and diphytanoyl phosphatidylcholine (DPhPC). Polymerization yielded BLMs that retain the fluidity required for alamethicin activity yet are stable for several days as compared to a few hours prior to polymerization. Thus, these polymerized, binary composition BLMs feature both fluidity and long-term stability.
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