ArticleGenetics2010
Functional genomics analysis of the Saccharomyces cerevisiae iron responsive transcription factor Aft1 reveals iron-independent functions.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Phenotypic heterogeneity follows a growth-viability tradeoff in response to amino acid identity.Nature communications · 2024Article
- Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii.Microbial cell factories · 2024Article
- Regulatory and pathogenic mechanisms in response to iron deficiency and excess in fungi.Microbial biotechnology · 2023Review
- Iron Limitation Restores Autophagy and Increases Lifespan in the Yeast Model of Niemann-Pick Type C1.International journal of molecular sciences · 2023Article
- Transcriptome profiling of transcription factors inFrontiers in microbiology · 2022Article
- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Fungal-Metal Interactions: A Review of Toxicity and Homeostasis.Journal of fungi (Basel, Switzerland) · 2021Review
- Self-Redirection of Metabolic Flux Toward Squalene and Ethanol Pathways by Engineered Yeast.Metabolites · 2020Article
- Yeast glutaredoxin, GRX4, functions as a glutathione S-transferase required for red ade pigment formation inJournal of biosciences · 2020Article
- The yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport system.Scientific reports · 2016Article
- Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast.The Journal of biological chemistry · 2016Article
- Implementation and assessment of a yeast orphan gene research project: involving undergraduates in authentic research experiences and progressing our understanding of uncharacterized open reading frames.Yeast (Chichester, England) · 2016Article
- A Signaling Lipid Associated with Alzheimer's Disease Promotes Mitochondrial Dysfunction.Scientific reports · 2016Article
- Using Gene Essentiality and Synthetic Lethality Information to Correct Yeast and CHO Cell Genome-Scale Models.Metabolites · 2015Article
- Rad9 interacts with Aft1 to facilitate genome surveillance in fragile genomic sites under non-DNA damage-inducing conditions in S. cerevisiae.Nucleic acids research · 2014Article
- Essential functions of iron-requiring proteins in DNA replication, repair and cell cycle control.Protein & cell · 2014Review
- HERC2 targets the iron regulator FBXL5 for degradation and modulates iron metabolism.The Journal of biological chemistry · 2014Article
- Genome-wide profiling of yeast DNA:RNA hybrid prone sites with DRIP-chip.PLoS genetics · 2014Article
- Article
- Iron-responsive transcription factor Aft1 interacts with kinetochore protein Iml3 and promotes pericentromeric cohesin.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low-iron conditions. In addition, Aft1 has been implicated in numerous cellular processes including cell-cycle progression and chromosome stability; however, it is unclear if all cellular effects of Aft1 are mediated through iron homeostasis. To further investigate the cellular processes affected by Aft1, we identified >70 deletion mutants that are sensitive to perturbations in AFT1 levels using genome-wide synthetic lethal and synthetic dosage lethal screens. Our genetic network reveals that Aft1 affects a diverse range of cellular processes, including the RIM101 pH pathway, cell-wall stability, DNA damage, protein transport, chromosome stability, and mitochondrial function. Surprisingly, only a subset of mutants identified are sensitive to extracellular iron fluctuations or display genetic interactions with mutants of iron regulon genes AFT2 or FET3. We demonstrate that Aft1 works in parallel with the RIM101 pH pathway and the role of Aft1 in DNA damage repair is mediated by iron. In contrast, through both directed studies and microarray transcriptional profiling, we show that the role of Aft1 in chromosome maintenance and benomyl resistance is independent of its iron regulatory role, potentially through a nontranscriptional mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.