ArticleThe Journal of biological chemistry2010
PfNT2, a permease of the equilibrative nucleoside transporter family in the endoplasmic reticulum of Plasmodium falciparum.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Genetic disruption of nucleoside transporter 4 reveals its critical roles in malaria parasite sporozoite functions.Pathogens and global health · 2023Article
- Continuous In Vitro Culture ofCells · 2023Article
- An Alternative Culture Medium for Continuous In Vitro Propagation of the Human PathogenPathogens (Basel, Switzerland) · 2022Article
- An Uninvited Seat at the Dinner Table: How Apicomplexan Parasites Scavenge Nutrients from the Host.Microorganisms · 2021Review
- AGIA Tag System for Ultrastructural Protein Localization Analysis in Blood-StageFrontiers in cellular and infection microbiology · 2021Article
- Identification via a Parallel Hit Progression Strategy of Improved Small Molecule Inhibitors of the Malaria Purine Uptake Transporter that InhibitACS infectious diseases · 2019Article
- Targeting Channels and Transporters in Protozoan Parasite Infections.Frontiers in chemistry · 2018Review
- Targeting the Plasmodium vivax equilibrative nucleoside transporter 1 (PvENT1) for antimalarial drug development.International journal for parasitology. Drugs and drug resistance · 2016Article
- Purine import into malaria parasites as a target for antimalarial drug development.Annals of the New York Academy of Sciences · 2015Review
- Yeast-based high-throughput screen identifies Plasmodium falciparum equilibrative nucleoside transporter 1 inhibitors that kill malaria parasites.ACS chemical biology · 2015Article
- Transport proteins of parasitic protists and their role in nutrient salvage.Frontiers in plant science · 2014Review
- Identification and functional analysis of the primary pantothenate transporter, PfPAT, of the human malaria parasite Plasmodium falciparum.The Journal of biological chemistry · 2013Article
- Malaria parasite type 4 equilibrative nucleoside transporters (ENT4) are purine transporters with distinct substrate specificity.The Biochemical journal · 2012Article
- Analysis of chloroquine resistance transporter (CRT) isoforms and orthologues in S. cerevisiae yeast.Biochemistry · 2011Article
- Nutrient transport and pathogenesis in selected parasitic protozoa.Eukaryotic cell · 2011Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The survival and proliferation of the obligate intracellular malaria parasite Plasmodium falciparum require salvage of essential purines from the host. Genetic studies have previously shown that the parasite plasma membrane purine permease, PfNT1, plays an essential function in the transport of all naturally occurring purine nucleosides and nucleobases across the parasite plasma membrane. Here, we describe an intracellular permease, PfNT2. PfNT2 is, like PfNT1, a member of the equilibrative nucleoside transporter family. Confocal and immunoelectron microscopic analyses of transgenic parasites harboring green fluorescent protein- or hemagglutinin-tagged PfNT2 demonstrated endoplasmic reticulum localization. This localization was confirmed by colocalization with the endoplasmic reticulum marker PfBiP. Using yeast as a surrogate system, we show that targeting PfNT2 to the plasma membrane of fui1Delta cells lacking the plasma membrane nucleoside transporter Fui1 confers sensitivity to the toxic nucleoside analog 5-fluorouridine. This study provides the first evidence of an intracellular purine permease in apicomplexan parasites and suggests a novel biological function for the parasite endoplasmic reticulum during malaria infection.
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