ArticleJournal of proteome research2010
Quantitative phosphoproteomic analysis of T cell receptor signaling in diabetes prone and resistant mice.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Effects of Txk‑mediated activation of NF‑κB signaling pathway on neurological deficit and oxidative stress after ischemia‑reperfusion in rats.Molecular medicine reports · 2021Article
- Investigation of post-translational modifications in type 2 diabetes.Clinical proteomics · 2018Review
- Lighting Up T Lymphocyte Signaling with Quantitative Phosphoproteomics.Frontiers in immunology · 2017Review
- CD5-mediated inhibition of TCR signaling proceeds normally in the absence of SHP-1.International journal of molecular medicine · 2016Article
- The current state of the art of quantitative phosphoproteomics and its applications to diabetes research.Expert review of proteomics · 2016Review
- Protein networks and activation of lymphocytes.Current opinion in immunology · 2015Review
- Coupling enrichment methods with proteomics for understanding and treating disease.Proteomics. Clinical applications · 2015Review
- Isobaric labeling-based relative quantification in shotgun proteomics.Journal of proteome research · 2014Review
- Analytical challenges translating mass spectrometry-based phosphoproteomics from discovery to clinical applications.Electrophoresis · 2014Review
- Phosphoproteome profiling of the macrophage response to different toll-like receptor ligands identifies differences in global phosphorylation dynamics.Journal of proteome research · 2014Article
- Quantitative phosphoproteomic profiling of fiber differentiation and initiation in a fiberless mutant of cotton.BMC genomics · 2014Article
- Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in MCF7 breast cancer cells.Molecular & cellular proteomics : MCP · 2014Article
- Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Article
- Quantitative analysis of global phosphorylation changes with high-resolution tandem mass spectrometry and stable isotopic labeling.Methods (San Diego, Calif.) · 2013Review
- Isobaric protein-level labeling strategy for serum glycoprotein quantification analysis by liquid chromatography-tandem mass spectrometry.Analytical chemistry · 2013Article
- Loss of T cell progenitor checkpoint control underlies leukemia initiation in Rag1-deficient nonobese diabetic mice.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Toward quantitative phosphotyrosine profiling in vivo.Seminars in cell & developmental biology · 2012Review
- Current challenges in software solutions for mass spectrometry-based quantitative proteomics.Amino acids · 2012Review
- Quantitative phosphoproteomic analysis reveals a role for serine and threonine kinases in the cytoskeletal reorganization in early T cell receptor activation in human primary T cells.Molecular & cellular proteomics : MCP · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Type 1 diabetes, in human patients and NOD mice, results from an immune attack on insulin-producing beta-cells of the pancreas by autoreactive T lymphocytes. In NOD mice, genetically controlled perturbations in the signaling pathways downstream of the antigen-specific T cell receptor (TCR) may be instrumental in the altered responses of T cells, manifest as inefficient induction of apoptosis after recognition of self-antigens in the thymus or as perturbed reactivity of mature T cells in peripheral organs. To map this signaling difference(s), we have used mass spectrometry-based quantitative phosphoproteomics to compare the activation of primary CD4(+) T cells of diabetes-prone NOD and -resistant B6.H2g7 mice. Immunoprecipitation and IMAC purification of tyrosine-phosphorylated peptides, combined with a stable-isotope iTRAQ labeling, enabled us to identify and quantify over 77 phosphorylation events in 54 different proteins downstream of TCR stimulation of primary CD4(+) T cells. This analysis showed a generally higher level of phosphotyrosine in activated NOD cells, as well as several phosphorylation sites that appeared to be differentially regulated in these two strains (involving TXK, CD5, PAG1, and ZAP-70). These data highlight the differences in signaling between CD4(+) T cell compartments of NOD and B6g7 mice and may underlie the dysregulation of T cells in NOD mice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.