ArticleMolecular genetics and genomics : MGG2010
Proteomic and transcriptomic characterization of a virulence-deficient phosphatidylcholine-negative Agrobacterium tumefaciens mutant.
Article in Molecular genetics and genomics : MGG, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Cryo-EM structure of the Agrobacterium tumefaciens T4SS-associated T-pilus reveals stoichiometric protein-phospholipid assembly.Structure (London, England : 1993) · 2023Article
- Recombinant and endogenous ways to produce methylated phospholipids in Escherichia coli.Applied microbiology and biotechnology · 2021Article
- ExoS/ChvI Two-Component Signal-Transduction System Activated in the Absence of Bacterial Phosphatidylcholine.Frontiers in plant science · 2021Article
- The RNase YbeY Is Vital for Ribosome Maturation, Stress Resistance, and Virulence of the Natural Genetic EngineerJournal of bacteriology · 2019Article
- Loss of PodJ in Agrobacterium tumefaciens Leads to Ectopic Polar Growth, Branching, and Reduced Cell Division.Journal of bacteriology · 2016Article
- Article
- Two separate modules of the conserved regulatory RNA AbcR1 address multiple target mRNAs in and outside of the translation initiation region.RNA biology · 2014Article
- Membrane lipids in Agrobacterium tumefaciens: biosynthetic pathways and importance for pathogenesis.Frontiers in plant science · 2014Review
- Profound impact of Hfq on nutrient acquisition, metabolism and motility in the plant pathogen Agrobacterium tumefaciens.PloS one · 2014Article
- Plant-Agrobacterium interaction mediated by ethylene and super-Agrobacterium conferring efficient gene transfer.Frontiers in plant science · 2014Article
- Increased 1-aminocyclopropane-1-carboxylate deaminase activity enhances Agrobacterium tumefaciens-mediated gene delivery into plant cells.MicrobiologyOpen · 2013Article
- A genome-wide survey of highly expressed non-coding RNAs and biological validation of selected candidates in Agrobacterium tumefaciens.PloS one · 2013Article
- A trapping approach reveals novel substrates and physiological functions of the essential protease FtsH in Escherichia coli.The Journal of biological chemistry · 2012Article
- Hfq influences multiple transport systems and virulence in the plant pathogen Agrobacterium tumefaciens.Journal of bacteriology · 2012Article
- Deep sequencing uncovers numerous small RNAs on all four replicons of the plant pathogen Agrobacterium tumefaciens.RNA biology · 2012Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylcholine (PC) is the most abundant phospholipid in eukaryotic membranes, whereas only a limited number of bacteria are able to synthesize PC. Intriguingly, many of the bacteria with PC-containing membranes interact with eukaryotic hosts. PC is one of the major membrane lipids in the phytopathogenic bacterium Agrobacterium tumefaciens. The presence of PC is critical for diverse cellular processes like motility, biofilm formation, stress resistance, and virulence. The exact role of PC in these processes is unknown. Here, we examined the global consequences of the complete loss of PC at the proteomic and transcriptomic levels. Both strategies validated the impaired virulence gene induction responsible for the virulence defect of the PC-deficient mutant. In addition, the proteomic approach revealed a limited subset of proteins with altered abundance including the reduced flagellar proteins FlaA and FlaB, which explains the motility defect of the PC mutant. At the whole-genome level, the loss of PC was correlated with altered expression of up to 13% of all genes, most encoding membrane or membrane-associated proteins and proteins with functions in the extracytoplasmic stress response. Our integrated analysis revealed that A. tumefaciens dynamically remodels its membrane protein composition in order to sustain normal growth in the absence of PC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.