ArticleThe Journal of biological chemistry2010
Stargazin modulates neuronal voltage-dependent Ca(2+) channel Ca(v)2.2 by a Gbetagamma-dependent mechanism.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- N-Type Ca Channel in Epileptic Syndromes and Epilepsy: A Systematic Review of Its Genetic Variants.International journal of molecular sciences · 2023Pooled it
- Encephalopathy-causing mutations in GβiScience · 2021Article
- Neurovascular coupling and oxygenation are decreased in hippocampus compared to neocortex because of microvascular differences.Nature communications · 2021Article
- miR-339 Promotes Hypoxia-Induced Neuronal Apoptosis and Impairs Cell Viability by Targeting FGF9/CACNG2 and Mediating MAPK Pathway in Ischemic Stroke.Frontiers in neurology · 2020Article
- Emerging roles for multifunctional ion channel auxiliary subunits in cancer.Cell calcium · 2019Review
- The DLGAP family: neuronal expression, function and role in brain disorders.Molecular brain · 2017Review
- Trafficking of neuronal calcium channels.Neuronal signaling · 2017Review
- Selective pressure modulation of synaptic voltage-dependent calcium channels-involvement in HPNS mechanism.Journal of cellular and molecular medicine · 2016Article
- Regulation of Ca(V)2 calcium channels by G protein coupled receptors.Biochimica et biophysica acta · 2013Review
- The DAO gene is associated with schizophrenia and interacts with other genes in the Taiwan Han Chinese population.PloS one · 2013Article
- The role of a voltage-dependent Ca2+ channel intracellular linker: a structure-function analysis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Trafficking and stability of voltage-gated calcium channels.Cellular and molecular life sciences : CMLS · 2012Review
- Genetic basis of pain variability: recent advances.Journal of medical genetics · 2012Review
- Susceptibility to chronic pain following nerve injury is genetically affected by CACNG2.Genome research · 2010Article
- G protein modulation of CaV2 voltage-gated calcium channels.Channels (Austin, Tex.)Review
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Authors and funding
4 authors.
Funding
Abstract
Loss of neuronal protein stargazin (gamma(2)) is associated with recurrent epileptic seizures and ataxia in mice. Initially, due to homology to the skeletal muscle calcium channel gamma(1) subunit, stargazin and other family members (gamma(3-8)) were classified as gamma subunits of neuronal voltage-gated calcium channels (such as Ca(V)2.1-Ca(V)2.3). Here, we report that stargazin interferes with G protein modulation of Ca(V)2.2 (N-type) channels expressed in Xenopus oocytes. Stargazin counteracted the Gbetagamma-induced inhibition of Ca(V)2.2 channel currents, caused either by coexpression of the Gbetagamma dimer or by activation of a G protein-coupled receptor. Expression of high doses of Gbetagamma overcame the effects of stargazin. High affinity Gbetagamma scavenger proteins m-cbetaARK and m-phosducin produced effects similar to stargazin. The effects of stargazin and m-cbetaARK were not additive, suggesting a common mechanism of action, and generally independent of the presence of the Ca(V)beta(3) subunit. However, in some cases, coexpression of Ca(V)beta(3) blunted the modulation by stargazin. Finally, the Gbetagamma-opposing action of stargazin was not unique to Ca(V)2.2, as stargazin also inhibited the Gbetagamma-mediated activation of the G protein-activated K(+) channel. Purified cytosolic C-terminal part of stargazin bound Gbetagamma in vitro. Our results suggest that the regulation by stargazin of biophysical properties of Ca(V)2.2 are not exerted by direct modulation of the channel but via a Gbetagamma-dependent mechanism.
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