ArticlePlant physiology2010
Characterization of the branched-chain amino acid aminotransferase enzyme family in tomato.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 105 citations in OpenAlex.
- Branched-chain amino acid catabolism initiates volatile synthesis in Gentiana triflora.Planta · 2025Article
- Capsaicin as a Microbiome Modulator: Metabolic Interactions and Implications for Host Health.Metabolites · 2025Review
- Fruits Produce Branched-Chain Esters Primarily from Newly Synthesized Precursors.Journal of agricultural and food chemistry · 2025Article
- Exogenous MeJA modulates postharvest tomato aroma by suppressing JAs-ethylene signaling crosstalk.Frontiers in plant science · 2025Article
- The amino acid permeaseHorticulture research · 2025Article
- Genome-Wide Association Studies Using 3VmrMLM Model Provide New Insights into Branched-Chain Amino Acid Contents in Rice Grains.Plants (Basel, Switzerland) · 2023Article
- Salt Stress-Related Mechanisms in Leaves of the Wild BarleyLife (Basel, Switzerland) · 2023Article
- DROUGHT-INDUCED BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE enhances drought tolerance in rice.Plant physiology · 2023Article
- The dissection of tomato flavor: biochemistry, genetics, and omics.Frontiers in plant science · 2023Review
- A metabolome and transcriptome survey to tap the dynamics of fruit prolonged shelf-life and improved quality within Greek tomato germplasm.Frontiers in plant science · 2023Article
- Targeted approaches to improve tomato fruit taste.Horticulture research · 2023Article
- Research progress on branched-chain amino acid aminotransferases.Frontiers in genetics · 2023Review
- Genome-wide association study of eigenvectors provides genetic insights into selective breeding for tomato metabolites.BMC biology · 2022Article
- Volatile Variation ofMolecules (Basel, Switzerland) · 2022Article
- Exogenous ABA promotes aroma biosynthesis of postharvest kiwifruit after low-temperature storage.Planta · 2022Article
- Regulation of the Leucine Metabolism inJournal of fungi (Basel, Switzerland) · 2022Article
- Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases.Molecules (Basel, Switzerland) · 2022Article
- Key Enzymes Involved in the Synthesis of Hops Phytochemical Compounds: From Structure, Functions to Applications.International journal of molecular sciences · 2021Review
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Branched-chain amino acids (BCAAs) are synthesized in plants from branched-chain keto acids, but their metabolism is not completely understood. The interface of BCAA metabolism lies with branched-chain aminotransferases (BCAT) that catalyze both the last anabolic step and the first catabolic step. In this study, six BCAT genes from the cultivated tomato (Solanum lycopersicum) were identified and characterized. SlBCAT1, -2, -3, and -4 are expressed in multiple plant tissues, while SlBCAT5 and -6 were undetectable. SlBCAT1 and -2 are located in the mitochondria, SlBCAT3 and -4 are located in chloroplasts, while SlBCAT5 and -6 are located in the cytosol and vacuole, respectively. SlBCAT1, -2, -3, and -4 were able to restore growth of Escherichia coli BCAA auxotrophic cells, but SlBCAT1 and -2 were less effective than SlBCAT3 and -4 in growth restoration. All enzymes were active in the forward (BCAA synthesis) and reverse (branched-chain keto acid synthesis) reactions. SlBCAT3 and -4 exhibited a preference for the forward reaction, while SlBCAT1 and -2 were more active in the reverse reaction. While overexpression of SlBCAT1 or -3 in tomato fruit did not significantly alter amino acid levels, an expression quantitative trait locus on chromosome 3, associated with substantially higher expression of Solanum pennellii BCAT4, did significantly increase BCAA levels. Conversely, antisense-mediated reduction of SlBCAT1 resulted in higher levels of BCAAs. Together, these results support a model in which the mitochondrial SlBCAT1 and -2 function in BCAA catabolism while the chloroplastic SlBCAT3 and -4 function in BCAA synthesis.
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