ArticleEukaryotic cell2010
Rotenone enhances the antifungal properties of staurosporine.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Staurosporine as an Antifungal Agent.International journal of molecular sciences · 2025Review
- Changes in the Biophysical Properties of the Cell Membrane Are Involved in the Response ofFrontiers in physiology · 2018Article
- Regulated Forms of Cell Death in Fungi.Frontiers in microbiology · 2017Review
- Neurospora crassa transcriptomics reveals oxidative stress and plasma membrane homeostasis biology genes as key targets in response to chitosan.Molecular bioSystems · 2016Article
- Activation of a TRP-like channel and intracellular Ca2+ dynamics during phospholipase-C-mediated cell death.Journal of cell science · 2014Article
- Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death inMicrobial cell (Graz, Austria) · 2014Article
- CZT-1 is a novel transcription factor controlling cell death and natural drug resistance in Neurospora crassa.G3 (Bethesda, Md.) · 2014Article
- Ten challenges on Cryptococcus and cryptococcosis.Mycopathologia · 2012Review
- Modulation of fungal sensitivity to staurosporine by targeting proteins identified by transcriptional profiling.Fungal genetics and biology : FG & B · 2011Article
- Synergistic growth inhibition of cancer cells harboring the RET/PTC1 oncogene by staurosporine and rotenone involves enhanced cell death.Journal of biosciences · 2011Article
- Involvement of p53 in cell death following cell cycle arrest and mitotic catastrophe induced by rotenone.Biochimica et biophysica acta · 2011Article
- The MAP kinase Pmk1 and protein kinase A are required for rotenone resistance in the fission yeast, Schizosaccharomyces pombe.Biochemical and biophysical research communications · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
We studied staurosporine-induced cell death in the filamentous fungus Neurospora crassa. The generation of reactive oxygen species during the process appears to be an important signaling event, since addition of the antioxidant glutathione prevents the effects of staurosporine on fungal growth. Selected mutants with mutations in respiratory chain complex I are extremely sensitive to the drug, stressing the involvement of complex I in programmed cell death. Following this finding, we determined that the complex I-specific inhibitor rotenone used in combination with staurosporine results in a synergistic and specific antifungal activity, likely through a concerted action on intracellular glutathione depletion. Paradoxically, the synergistic antifungal activity of rotenone and staurosporine is observed in N. crassa complex I mutants and in Saccharomyces cerevisiae, which lacks complex I. In addition, it is not observed when other complex I inhibitors are used instead of rotenone. These results indicate that the rotenone effect is independent of complex I inhibition. The combination of rotenone and staurosporine is effective against N. crassa as well as against the common pathogens Aspergillus fumigatus and Candida albicans, pointing to its usefulness as an antifungal agent.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.