ArticleEnvironmental health perspectives2010
Formation of covalently bound protein adducts from the cytotoxicant naphthalene in nasal epithelium: species comparisons.
Article in Environmental health perspectives, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 23 citations in OpenAlex.
- Naphthalene: MAK Value Documentation, addendum - Translation of the German version from 2022.The MAK collection for occupational health and safety · 2025Article
- Naphthalene genotoxicity: DNA adducts in primate and mouse airway explants.Toxicology letters · 2019Article
- Naphthalene DNA Adduct Formation and Tolerance in the Lung.Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms · 2019Article
- Hypothesis-based weight-of-evidence evaluation and risk assessment for naphthalene carcinogenesis.Critical reviews in toxicology · 2016Review
- Alterations in the proteome of the respiratory tract in response to single and multiple exposures to naphthalene.Proteomics · 2015Article
- Characterization of model peptide adducts with reactive metabolites of naphthalene by mass spectrometry.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNaphthalene is a volatile hydrocarbon that causes dose-, species-, and cell type-dependent cytotoxicity after acute exposure and hyperplasia/neoplasia after lifetime exposures in rodents. Toxicity depends on metabolic activation, and reactive metabolite binding correlates with tissue and site susceptibility.
objectivesWe compared proteins adducted in nasal epithelium from rats and rhesus macaques in vitro.
methodsAdducted proteins recovered from incubations of nasal epithelium and 14C-naphthalene were separated by two-dimensional (2D) gel electrophoresis and imaged to register radioactive proteins. We identified proteins visualized by silver staining on complementary non-radioactive gels by peptide mass mapping.
resultsThe levels of reactive metabolite binding in incubations of rhesus ethmo-turbinates and maxillo-turbinates are similar to those in incubations of target tissues, including rat septal/-olfactory regions and murine dissected airway incubations. We identified 40 adducted spots from 2D gel separations of rat olfactory epithelial proteins; 22 of these were non-redundant. In monkeys, we identified 19 spots by mass spectrometry, yielding three non-redundant identifications. Structural proteins (actin/tubulin) were prominent targets in both species.
conclusionsIn this study we identified potential target proteins that may serve as markers closely associated with toxicity. The large differences in previously reported rates of naphthalene metabolism to water-soluble metabolites in dissected airways from mice and monkeys are not reflected in similar differences in covalent adduct formation in the nose. This raises concerns that downstream metabolic/biochemical events are very similar between the rat, a known target for naphthalene toxicity and tumorigenicity, and the rhesus macaque, a species similar to the human.
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