ArticleResearch in microbiology2010
Bacteroides fragilis RecA protein overexpression causes resistance to metronidazole.
Article in Research in microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
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- The Ambivalent Nature ofToxins · 2025Review
- Vaginal pharmacomicrobiomics modulates risk of persistent and recurrent bacterial vaginosis.NPJ biofilms and microbiomes · 2025Review
- Coordination of prophage and global regulator leads to high enterotoxin production in staphylococcal food poisoning-associated lineage.Microbiology spectrum · 2024Article
- Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance ofAntibiotics (Basel, Switzerland) · 2024Article
- Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates.Microorganisms · 2023Review
- Time for Some Group Therapy: Update on Identification, Antimicrobial Resistance, Taxonomy, and Clinical Significance of the Bacteroides fragilis Group.Journal of clinical microbiology · 2022Review
- Chromosomal Resistance to Metronidazole in Clostridioides difficile Can Be Mediated by Epistasis between Iron Homeostasis and Oxidoreductases.Antimicrobial agents and chemotherapy · 2020Article
- Antimicrobial Susceptibility and Clonality of Vaginally Derived Multidrug-ResistantAntimicrobial agents and chemotherapy · 2020Article
- Article
- Generation of Markerless Deletions in the Nosocomial PathogenApplied and environmental microbiology · 2019Article
- Proteomic Signatures ofCells · 2018Article
- Three metronidazole-resistant Prevotella bivia strains harbour a mobile element, encoding a novel nim gene, nimK, and an efflux small MDR transporter.The Journal of antimicrobial chemotherapy · 2018Article
- Article
- Hot-spot identification on a broad class of proteins and RNA suggest unifying principles of molecular recognition.PloS one · 2017Article
- nim gene-independent metronidazole-resistant Bacteroides fragilis in surgical site infections.GMS hygiene and infection control · 2017Article
- Exploratory Investigation ofFrontiers in microbiology · 2016Article
- Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis.The Journal of antimicrobial chemotherapy · 2014Article
- The recA operon: A novel stress response gene cluster in Bacteroides fragilis.Research in microbiology · 2014Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Bacteroides fragilis is a human gut commensal and an opportunistic pathogen causing anaerobic abscesses and bacteraemias which are treated with metronidazole (Mtz), a DNA damaging agent. This study examined the role of the DNA repair protein, RecA, in maintaining endogenous DNA stability and its contribution to resistance to Mtz and other DNA damaging agents. RT-PCR of B. fragilis genomic DNA showed that the recA gene was co-transcribed as an operon together with two upstream genes, putatively involved in repairing oxygen damage. A B. fragilis recA mutant was generated using targeted gene inactivation. Fluorescence microscopy using DAPI staining revealed increased numbers of mutant cells with reduced intact double-stranded DNA. Alkaline gel electrophoresis of the recA mutant DNA showed increased amounts of strand breaks under normal growth conditions, and the recA mutant also showed less spontaneous mutagenesis relative to the wild type strain. The recA mutant was sensitive to Mtz, ultraviolet light and hydrogen peroxide. A B. fragilis strain overexpressing the RecA protein exhibited increased resistance to Mtz compared to the wild type. This is the first study to show that overexpression of a DNA repair protein in B. fragilis increases Mtz resistance. This represents a novel drug resistance mechanism in this bacterium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.