ArticleJournal of molecular biology2010
Consensus protein design without phylogenetic bias.
Article in Journal of molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed.
- Evolutionary Rate Variation at Predicted PTM Sites Reveals Localized Host-Associated Patterns in Influenza A Virus.Genome biology and evolution · 2026Article
- PyTEA-O: a Python implementation of Two-Entropies Analysis for protein sequence variation analysis.Bioinformatics (Oxford, England) · 2026Article
- Thermostability enhancement and insight of L-asparaginase from Mycobacterium sp. via consensus-guided engineering.Applied microbiology and biotechnology · 2023Article
- Therapeutic enzyme engineering using a generative neural network.Scientific reports · 2022Article
- Catalytic mechanism of ancestral L-lysine oxidase assigned by sequence data mining.The Journal of biological chemistry · 2021Article
- Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials.Nature communications · 2021Article
- Protein Engineering Approaches to Enhance Fungal Laccase Production inInternational journal of molecular sciences · 2021Article
- Article
- Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis.Microbial cell factories · 2020Article
- The use of consensus sequence information to engineer stability and activity in proteins.Methods in enzymology · 2020Article
- Consensus Design of an Evolved High-Redox Potential Laccase.Frontiers in bioengineering and biotechnology · 2020Article
- Following the Evolutionary Track of a Highly Specific l-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes.Applied and environmental microbiology · 2019Article
- Validation and Stabilization of a Prophage Lysin of Clostridium perfringens by Using Yeast Surface Display and Coevolutionary Models.Applied and environmental microbiology · 2019Article
- Monomerization of far-red fluorescent proteins.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The CaspBase: a curated database for evolutionary biochemical studies of caspase functional divergence and ancestral sequence inference.Protein science : a publication of the Protein Society · 2018Article
- Bacterially expressed HIV-1 gp120 outer-domain fragment immunogens with improved stability and affinity for CD4-binding site neutralizing antibodies.The Journal of biological chemistry · 2018Article
- Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120.BMC biotechnology · 2018Article
- Evaluation of affibody charge modification identified by synthetic consensus design in molecular PET imaging of epidermal growth factor receptor.Molecular systems design & engineering · 2018Article
- Stabilization of a nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator yields insight into disease-causing mutations.The Journal of biological chemistry · 2017Article
- Effects of point mutations on the thermostability of B. subtilis lipase: investigating nonadditivity.Journal of computer-aided molecular design · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Consensus design is an appealing strategy for the stabilization of proteins. It exploits amino acid conservation in sets of homologous proteins to identify likely beneficial mutations. Nevertheless, its success depends on the phylogenetic diversity of the sequence set available. Here, we show that randomization of a single protein represents a reliable alternative source of sequence diversity that is essentially free of phylogenetic bias. A small number of functional protein sequences selected from binary-patterned libraries suffice as input for the consensus design of active enzymes that are easier to produce and substantially more stable than individual members of the starting data set. Although catalytic activity correlates less consistently with sequence conservation in these extensively randomized proteins, less extreme mutagenesis strategies might be adopted in practice to augment stability while maintaining function.
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