ArticleBMC genomics2010
An EST resource for tilapia based on 17 normalized libraries and assembly of 116,899 sequence tags.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Genome-Wide Association Study and RNA-Seq Analysis Uncover Candidate Genes Controlling Growth Traits in Red Tilapia (International journal of molecular sciences · 2025Article
- Mapping QTL for Sex and Growth Traits in Salt-Tolerant Tilapia (Oreochromis spp. X O. mossambicus).PloS one · 2016Article
- Signatures of selection in tilapia revealed by whole genome resequencing.Scientific reports · 2015Article
- Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles).Scientific reports · 2015Article
- Nutritional status and growth hormone regulate insulin-like growth factor binding protein (igfbp) transcripts in Mozambique tilapia.General and comparative endocrinology · 2014Article
- Article
- Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptation.International journal of genomics · 2014Article
- Article
- Evolution of genomic structural variation and genomic architecture in the adaptive radiations of African cichlid fishes.Frontiers in genetics · 2014Article
- Whole genome comparative analysis of channel catfish (Ictalurus punctatus) with four model fish species.BMC genomics · 2013Article
- Comparative genomic analysis of catfish linkage group 8 reveals two homologous chromosomes in zebrafish and other teleosts with extensive inter-chromosomal rearrangements.BMC genomics · 2013Article
- Deep sequencing for de novo construction of a marine fish (Sparus aurata) transcriptome database with a large coverage of protein-coding transcripts.BMC genomics · 2013Article
- A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity.PloS one · 2013Article
- Article
- Integrating cytogenetics and genomics in comparative evolutionary studies of cichlid fish.BMC genomics · 2012Article
- Transcriptomic characterization of the larval stage in gilthead seabream (Sparus aurata) by 454 pyrosequencing.Marine biotechnology (New York, N.Y.) · 2012Article
- Transcriptome characterization via 454 pyrosequencing of the annelid Pristina leidyi, an emerging model for studying the evolution of regeneration.BMC genomics · 2012Article
- A high-resolution map of the Nile tilapia genome: a resource for studying cichlids and other percomorphs.BMC genomics · 2012Article
- Article
- Linkage and Physical Mapping of Sex Region on LG23 of Nile Tilapia (Oreochromis niloticus).G3 (Bethesda, Md.) · 2012Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
backgroundLarge collections of expressed sequence tags (ESTs) are a fundamental resource for analysis of gene expression and annotation of genome sequences. We generated 116,899 ESTs from 17 normalized and two non-normalized cDNA libraries representing 16 tissues from tilapia, a cichlid fish widely used in aquaculture and biological research.
resultsThe ESTs were assembled into 20,190 contigs and 36,028 singletons for a total of 56,218 unique sequences and a total assembled length of 35,168,415 bp. Over the whole project, a unique sequence was discovered for every 2.079 sequence reads. 17,722 (31.5%) of these unique sequences had significant BLAST hits (e-value < 10(-10)) to the UniProt database.
conclusionNormalization of the cDNA pools with double-stranded nuclease allowed us to efficiently sequence a large collection of ESTs. These sequences are an important resource for studies of gene expression, comparative mapping and annotation of the forthcoming tilapia genome sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.