ArticleBiochemistry2010
Control of erythrocyte membrane-skeletal cohesion by the spectrin-membrane linkage.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Repurposing Tyrosine Kinase Inhibitors for Sickle Cell Disease: Focus on Band 3 Phosphorylation.Biomedicines · 2026Review
- Red cell shape regulation by band 3-ankyrin-spectrin linkage: implications for clinical severity of bovine hereditary spherocytosis.Blood red cells & iron · 2025Article
- Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes.Membranes · 2023Article
- Aging Injury Impairs Structural Properties and Cell Signaling in Human Red Blood Cells; Açaì Berry Is a Keystone.Antioxidants (Basel, Switzerland) · 2023Article
- Morphological Characteristics, Hemoglobin Content, and Membrane Mechanical Properties of Red Blood Cell Delivery Systems.ACS applied materials & interfaces · 2022Article
- Topological Relationships Cytoskeleton-Membrane Nanosurface-Morphology as a Basic Mechanism of Total Disorders of RBC Structures.International journal of molecular sciences · 2022Article
- Two-step process of cytoskeletal structural damage during long-term storage of packed red blood cells.Blood transfusion = Trasfusione del sangue · 2021Article
- Correlated flickering of erythrocytes membrane observed with dual time resolved membrane fluctuation spectroscopy under different D-glucose concentrations.Scientific reports · 2021Article
- Differential dielectroscopic data on the relation of erythrocyte membrane skeleton to erythrocyte deformability and flicker.European biophysics journal : EBJ · 2021Article
- Multiple Functions of Spectrin: Convergent Effects.The Journal of membrane biology · 2020Review
- A new molecular link between defective autophagy and erythroid abnormalities in chorea-acanthocytosis.Blood · 2016Article
- Red Blood Cell Homeostasis: Pharmacological Interventions to Explore Biochemical, Morphological and Mechanical Properties.Frontiers in molecular biosciences · 2016Article
- Processing of Plasmodium falciparum Merozoite Surface Protein MSP1 Activates a Spectrin-Binding Function Enabling Parasite Egress from RBCs.Cell host & microbe · 2015Article
- Particle Simulation of Oxidation Induced Band 3 Clustering in Human Erythrocytes.PLoS computational biology · 2015Article
- Transformation of membrane nanosurface of red blood cells under hemin action.Scientific reports · 2014Article
- Effect of radiographic contrast media on the spectrin/band3-network of the membrane skeleton of erythrocytes.PloS one · 2014Article
- Erythrocyte stiffness during morphological remodeling induced by carbon ion radiation.PloS one · 2014Article
- N-ethylmaleimide activates a Cl(-)-independent component of K(+) flux in mouse erythrocytes.Blood cells, molecules & diseases · 2013Article
- The effect of alcohols on red blood cell mechanical properties and membrane fluidity depends on their molecular size.PloS one · 2013Article
- Intruders below the radar: molecular pathogenesis of Bartonella spp.Clinical microbiology reviews · 2012Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Spectrin tetramer is the major structural member of the membrane-associated skeletal network of red cells. We show here that disruption of the spectrin-ankyrin-band 3 link to the membrane leads to dissociation of a large proportion of the tetramers into dimers. Noncovalent perturbation of the linkage was induced by a peptide containing the ankyrin-binding site of the spectrin beta-chain, and covalent perturbation by treatment with the thiol reagent, N-ethylmaleimide (NEM). This reagent left the intrinsic self-association capacity of the spectrin dimers unaffected and disturbed only the ankyrin-band 3 interaction. The dissociation of spectrin tetramers on the membrane into functional dimers was confirmed by the binding of a spectrin peptide directed against the self-association sites. Dissociation of the tetramers resulted, we infer, from detachment of the proximal ends of the constituent dimers from the membrane, thereby reducing their proximity to one another and thus weakening their association. The measured affinity of the interaction of the peptides with the free dimer ends on the membrane permits an estimate of the equilibrium between intact and dissociated tetramers on the native membrane. This indicates that in the physiological state the equilibrium proportion of the dissociated tetramers may be as high as 5-10%. These findings enabled us to identify an additional important functional role for the spectrin-ankyrin-band 3 link in regulating spectrin self-association in the red cell membrane.
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