ArticleBiochemistry2010
Mapping of lysine methylation and acetylation in core histones of Neurospora crassa.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Nuclear genome organization in fungi: from gene folding to Rabl chromosomes.FEMS microbiology reviews · 2023Article
- A phylogenetic and proteomic reconstruction of eukaryotic chromatin evolution.Nature ecology & evolution · 2022Article
- Post-Translational Modifications of Histones Are Versatile Regulators of Fungal Development and Secondary Metabolism.Toxins · 2022Review
- TheFrontiers in cellular and infection microbiology · 2018Article
- H3K36ac Is an Evolutionary Conserved Plant Histone Modification That Marks Active Genes.Plant physiology · 2016Article
- KdmA, a histone H3 demethylase with bipartite function, differentially regulates primary and secondary metabolism in Aspergillus nidulans.Molecular microbiology · 2015Article
- α-N-methylation of damaged DNA-binding protein 2 (DDB2) and its function in nucleotide excision repair.The Journal of biological chemistry · 2014Article
- Identification of novel α-n-methylation of CENP-B that regulates its binding to the centromeric DNA.Journal of proteome research · 2013Article
- Substitutions in the amino-terminal tail of neurospora histone H3 have varied effects on DNA methylation.PLoS genetics · 2011Article
- Breaking the histone code with quantitative mass spectrometry.Expert review of proteomics · 2011Review
- Histone variant innovation in a rapidly evolving chordate lineage.BMC evolutionary biology · 2011Article
- Extensive and varied modifications in histone H2B of wild-type and histone deacetylase 1 mutant Neurospora crassa.Biochemistry · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Core histones are susceptible to a variety of post-translational modifications (PTMs), among which methylation and acetylation play critical roles in various chromatin-dependent processes. The nature and biological functions of these PTMs have been extensively studied in plants, animals, and yeasts. In contrast, the histone modifications in Neurospora crassa, a convenient model organism for multicellular eukaryotes, remained largely undefined. In this study, we used several mass spectrometric techniques, coupled with HPLC separation and multiple-protease digestion, to identify the methylation and acetylation sites in core histones isolated from Neurospora. Electron transfer dissociation (ETD) was employed to fragment the heavily modified long N-terminal peptides. In addition, accurate mass measurement of fragment ions allowed for unambiguous differentiation of acetylation from trimethylation. Many modification sites conserved in other organisms were identified in Neurospora. In addition, some unique modification sites in histone H2B, including N-terminal alpha methylation, methylation at K3, and acetylation at K19, K28, and K29, were observed. Our analysis provides a potentially comprehensive picture of methylation and acetylation of core histones in Neurospora, which should serve as a foundation for future studies of the function of histone PTMs in this model organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.