ArticlePlant physiology2010
Novel hydroxycinnamoyl-coenzyme A quinate transferase genes from artichoke are involved in the synthesis of chlorogenic acid.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 151 citations in OpenAlex.
- Genome-Wide Datasets of Chicories (International journal of molecular sciences · 2023Pooled it
- Advances in the Function Roles of Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferases: A Key Enzyme Linking Phenylpropanoid Metabolism to Plant Terrestrial Adaptation.Plants (Basel, Switzerland) · 2026Review
- Genome-wide analysis of BAHD gene family and in vivo characterization of HQT-like genes involved in chlorogenic acid synthesis in tobacco (Nicotiana tabacum L.).BMC plant biology · 2026Article
- Cloning and Characterization ofGenes · 2026Article
- Untargeted Metabolomics Reveals the Effect of Carbon Dots on Improving the Shelf Life of Postharvest Goji Berries (Foods (Basel, Switzerland) · 2025Article
- Combining Stable Isotope Labeling and Candidate Substrate-Product Pair Networks Reveals Lignan, Oligolignol, and Chicoric Acid Biosynthesis in Flax Seedlings (Plants (Basel, Switzerland) · 2025Article
- Biosynthetic Mechanisms of Plant Chlorogenic Acid from a Microbiological Perspective.Microorganisms · 2025Review
- Integrative analysis of genome-wide association studies of polyphenols in apple fruits identifies the MdDof2.4Horticulture research · 2025Article
- A reduced vernalization requirement is a key component of the early-bolting trait in globe artichoke (iScience · 2024Article
- Identification ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Exploring the metabolic and physiological roles ofFrontiers in plant science · 2023Article
- Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatasInternational journal of molecular sciences · 2022Article
- The use of ecological analytical tools as an unconventional approach for untargeted metabolomics data analysis: the case of Cecropia obtusifolia and its adaptive responses to nitrate starvation.Functional & integrative genomics · 2022Article
- Polyphenolic Compound Variation in Globe Artichoke Cultivars as Affected by Fertilization and Biostimulants Application.Plants (Basel, Switzerland) · 2022Article
- Defining key metabolic roles in osmotic adjustment and ROS homeostasis in the recretohalophyte Karelinia caspia under salt stress.Physiologia plantarum · 2022Article
- Integrative analysis of the metabolome and transcriptome reveals the molecular mechanism of chlorogenic acid synthesis in peach fruit.Frontiers in nutrition · 2022Article
- Towards a Cardoon (International journal of molecular sciences · 2021Article
- Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity.The Plant journal : for cell and molecular biology · 2021Review
- Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field.International journal of molecular sciences · 2021Review
- Versatility in acyltransferase activity completes chicoric acid biosynthesis in purple coneflower.Nature communications · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Artichoke (Cynara cardunculus subsp. scolymus) extracts have high antioxidant capacity, due primarily to flavonoids and phenolic acids, particularly chlorogenic acid (5-caffeoylquinic acid [CGA]), dicaffeoylquinic acids, and caffeic acid, which are abundant in flower bracts and bioavailable to humans in the diet. The synthesis of CGA can occur following different routes in plant species, and hydroxycinnamoyl-coenzyme A transferases are important enzymes in these pathways. Here, we report on the isolation and characterization of two novel genes both encoding hydroxycinnamoyl-coenzyme A quinate transferases (HQT) from artichoke. The recombinant proteins (HQT1 and HQT2) were assayed after expression in Escherichia coli, and both showed higher affinity for quinate over shikimate. Their preferences for acyl donors, caffeoyl-coenzyme A or p-coumaroyl-coenzyme A, were examined. Modeling and docking analyses were used to propose possible pockets and residues involved in determining substrate specificities in the HQT enzyme family. Quantitative real-time polymerase chain reaction analysis of gene expression indicated that HQT1 might be more directly associated with CGA content. Transient and stable expression of HQT1 in Nicotiana resulted in a higher production of CGA and cynarin (1,3-dicaffeoylquinic acid). These findings suggest that several isoforms of HQT contribute to the synthesis of CGA in artichoke according to physiological needs and possibly following various metabolic routes.
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