ArticleThe Journal of molecular diagnostics : JMD2010
Single PCR multiplex SNaPshot reaction for detection of eleven blood group nucleotide polymorphisms: optimization, validation, and one year of routine clinical use.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
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- An Assay for Variable Fragment Length Allele-Specific Genotyping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Blood Group Testing.Frontiers in medicine · 2022Review
- Blood group typing from whole-genome sequencing data.PloS one · 2020Article
- Molecular profiling of non-small cell lung cancer.PloS one · 2020Article
- Extended Donor Typing by Pooled Capillary Electrophoresis: Impact in a Routine Setting.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2018Article
- Associations between thromboxane A synthase 1 gene polymorphisms and the risk of ischemic stroke in a Chinese Han population.Neural regeneration research · 2018Article
- Identification of Forensically Important Calliphoridae and Sarcophagidae Species Collected in Korea Using SNaPshot Multiplex System Targeting the Cytochrome c Oxidase Subunit I Gene.BioMed research international · 2018Article
- Addressing the challenges of applying precision oncology.NPJ precision oncology · 2017Review
- Serum and red-blood-cell folate demonstrate differential associations with BMI in pregnant women.Public health nutrition · 2016Article
- HLA-DPB1 Variant Effect on Hepatitis B Virus Clearance and Liver Cirrhosis Development Among Southwest Chinese Population.Hepatitis monthly · 2014Article
- Polymorphisms in CISH gene are associated with persistent hepatitis B virus infection in Han Chinese population.PloS one · 2014Article
- A new strategy to identify rare blood donors: single polymerase chain reaction multiplex SNaPshot reaction for detection of 16 blood group alleles.Blood transfusion = Trasfusione del sangue · 2014Article
- Association of interleukin-33 gene single nucleotide polymorphisms with ischemic stroke in north Chinese population.BMC medical genetics · 2013Article
- Association between microRNA polymorphisms and humoral immunity to hepatitis B vaccine.Human vaccines & immunotherapeutics · 2013Article
- Improved allele-specific PCR technique for Kidd blood group genotyping.Journal of clinical laboratory analysis · 2013Article
- Association of Fcγ receptor IIB polymorphism with cryptococcal meningitis in HIV-uninfected Chinese patients.PloS one · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemagglutination-based assays have several clinical shortcomings. To overcome this difficulty, we have developed a multiplex-PCR SNaPshot assay adapted to the Southern French population, which includes individuals from sub-Saharan Africa and the Comoros archipelago. Single nucleotide polymorphisms (SNPs) associated with clinically relevant blood antigens as well as with null phenotypes were profiled (i.e., K/k, Fy(a)/Fy(b)/Fy(bw)/Fy(null), S/s/U-/U+(var), Jk(a)/Jk(b), Do(a)/Do(b), Yt(a)/Yt(b), and Co(a)/Co(b)). A single multiplex-PCR reaction was used to amplify nine gene regions encompassing 11 SNPs. Identification was obtained by incorporation of the complementary dye-conjugated single base at the 3' end of each probe primer annealed proximal to the target SNP. After optimization, the SNaPshot assay was validated with 265 known allele or phenotype pairs. Results were found fully concordant with those of hemagglutination, allele-specific PCR, and/or sequencing. The assay was then evaluated on 227 blood samples in a clinical context. A total of 203 derived-phenotypes were generated, including 82 atypical phenotypes [i.e., Fy(b+(w)) (n = 32); K(+) (n = 22); Co(b+) (n = 8); Yt(b+) (n = 18); S-s+U+(var) (n = 2), 105 null phenotypes, i.e., Fy(a-b-) (n = 97); S-s-U- (n = 6); S-s-U+(var) (n = 2)] and sixteen Fy-positive samples carried a FY*Fy allele. The findings show that this assay can provide a low-cost and fast genotyping tool well adapted to local ethnically mixed populations.
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