ArticleThe Journal of biological chemistry2010
J domain co-chaperone specificity defines the role of BiP during protein translocation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Genome-Wide Identification and Analysis of the Hsp40/J-Protein Family Reveals Its Role in Soybean (Genes · 2023Article
- The ER Protein Translocation Channel Subunit Sbh1 Controls Virulence of Cryptococcus neoformans.mBio · 2023Article
- Pathway engineering facilitates efficient protein expression in Pichia pastoris.Applied microbiology and biotechnology · 2022Review
- Multivalent protein-protein interactions are pivotal regulators of eukaryotic Hsp70 complexes.Cell stress & chaperones · 2022Review
- Revealing functional insights into ER proteostasis through proteomics and interactomics.Experimental cell research · 2021Review
- UniProt-Related Documents (UniReD): assisting wet lab biologists in their quest on finding novel counterparts in a protein network.NAR genomics and bioinformatics · 2020Article
- N-glycosylation Triggers a Dual Selection Pressure in Eukaryotic Secretory Proteins.Scientific reports · 2017Article
- Neurons Export Extracellular Vesicles Enriched in Cysteine String Protein and Misfolded Protein Cargo.Scientific reports · 2017Article
- RNA modification enzyme TruB is a tRNA chaperone.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Baculovirus-mediated GCRV vp7 and vp6 genes expression in silkworm and grass carp.Molecular biology reports · 2016Article
- Analysis of COPII Vesicles Indicates a Role for the Emp47-Ssp120 Complex in Transport of Cell Surface Glycoproteins.Traffic (Copenhagen, Denmark) · 2016Article
- Article
- Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity.The Journal of clinical investigation · 2015Article
- How hsp70 molecular machines interact with their substrates to mediate diverse physiological functions.Journal of molecular biology · 2015Review
- Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro.The Journal of biological chemistry · 2014Article
- Viral vaccines for farmed finfish.Virusdisease · 2014Review
- Orchestration of secretory protein folding by ER chaperones.Biochimica et biophysica acta · 2013Review
- Characterization of an M28 metalloprotease family member residing in the yeast vacuole.FEMS yeast research · 2013Article
- ERdj3 regulates BiP occupancy in living cells.Journal of cell science · 2013Article
- Hsp70 protein complexes as drug targets.Current pharmaceutical design · 2013Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Hsp70 chaperones can potentially interact with one of several J domain-containing Hsp40 co-chaperones to regulate distinct cellular processes. However, features within Hsp70s that determine Hsp40 specificity are undefined. To investigate this question, we introduced mutations into the ER-lumenal Hsp70, BiP/Kar2p, and found that an R217A substitution in the J domain-interacting surface of BiP compromised the physical and functional interaction with Sec63p, an Hsp40 required for ER translocation. In contrast, interaction with Jem1p, an Hsp40 required for ER-associated degradation, was unaffected. Moreover, yeast expressing R217A BiP exhibited defects in translocation but not in ER-associated degradation. Finally, the genetic interactions of the R217A BiP mutant were found to correlate with those of known translocation mutants. Together, our results indicate that residues within the Hsp70 J domain-interacting surface help confer Hsp40 specificity, in turn influencing distinct chaperone-mediated cellular activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.