ArticleMicrobiology (Reading, England)2010
Role of Hfq in iron-dependent and -independent gene regulation in Neisseria meningitidis.
Article in Microbiology (Reading, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the EnteropathogenInternational journal of molecular sciences · 2023Article
- Control ofMicroorganisms · 2020Review
- Regulatory RNAs in Virulence and Host-Microbe Interactions.Microbiology spectrum · 2018Review
- Bacterial Iron Homeostasis Regulation by sRNAs.Microbiology spectrum · 2018Review
- Regulation ofFEBS open bio · 2017Article
- Neisseria genomics: current status and future perspectives.Pathogens and disease · 2017Review
- The primary transcriptome of Neisseria meningitidis and its interaction with the RNA chaperone Hfq.Nucleic acids research · 2017Article
- The Hfq regulon ofFEBS open bio · 2017Article
- UnravelingF1000Research · 2017Review
- Article
- How do base-pairing small RNAs evolve?FEMS microbiology reviews · 2015Review
- Edwardsiella tarda Hfq: impact on host infection and global protein expression.Veterinary research · 2014Article
- Article
- Article
- The role of the RNA chaperone Hfq in Haemophilus influenzae pathogenesis.BMC microbiology · 2013Article
- Fur-mediated global regulatory circuits in pathogenic Neisseria species.Journal of bacteriology · 2012Review
- The zinc-responsive regulon of Neisseria meningitidis comprises 17 genes under control of a Zur element.Journal of bacteriology · 2012Article
- Analysis of the regulated transcriptome of Neisseria meningitidis in human blood using a tiling array.Journal of bacteriology · 2012Article
- Fur-mediated activation of gene transcription in the human pathogen Neisseria gonorrhoeae.Journal of bacteriology · 2012Article
- The RNA chaperone Hfq is important for growth and stress tolerance in Francisella novicida.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In Neisseria meningitidis, iron-responsive gene regulation is mediated primarily by the ferric uptake regulator (Fur) protein. When complexed with iron, Fur represses gene expression by preventing transcription initiation. Fur can also indirectly activate gene expression via the repression of regulatory small RNAs (sRNA). One such Fur- and iron-regulated sRNA, NrrF, was previously identified in N. meningitidis and shown to repress expression of the sdhA and sdhC genes encoding subunits of the succinate dehydrogenase complex. In the majority of Gram-negative bacteria, sRNA-mediated regulation requires a cofactor RNA-binding protein (Hfq) for proper gene regulation and stabilization. In this study, we examined the role of Hfq in NrrF-mediated regulation of the succinate dehydrogenase genes in N. meningitidis and the effect of an hfq mutation on iron-responsive gene regulation more broadly. We first demonstrated that the stability of NrrF, as well as the regulation of sdhC and sdhA in vivo, was unaltered in the hfq mutant. Secondly, we established that iron-responsive gene regulation of the Fur-regulated sodB gene was dependent on Hfq. Finally, we demonstrated that in N. meningitidis, Hfq functions in a global manner to control expression of many ORFs and intergenic regions via iron-independent mechanisms. Collectively these studies demonstrate that in N. meningitidis, iron- and NrrF-mediated regulation of sdhC and sdhA can occur independently of Hfq, although Hfq functions more globally to control regulation of other N. meningitidis genes primarily by iron-independent mechanisms.
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