ArticleBiotechnology letters2010
Flow cytometric fluorescence pulse width analysis of etoposide-induced nuclear enlargement in HCT116 cells.
Article in Biotechnology letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Estimating particle size and velocity from fluorescence pulses: A practical validation study of flow cytometry signals analysis.PloS one · 2026Article
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- Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results.Membranes · 2021Review
- The Role of Cholesterol on Triterpenoid Saponin-Induced Endolysosomal Escape of a Saporin-Based Immunotoxin.International journal of molecular sciences · 2020Article
- A Flow Cytometric Method to Quantify the Endosomal Escape of a Protein Toxin to the Cytosol of Target Cells.Pharmaceutical research · 2019Article
- Bleomycin Inhibits Proliferation via Schlafen-Mediated Cell Cycle Arrest in Mouse Alveolar Epithelial Cells.Tuberculosis and respiratory diseases · 2019Article
- High Throughput Analysis of Golgi Structure by Imaging Flow Cytometry.Scientific reports · 2017Article
- The Hydroxyl at Position C1 of Genipin Is the Active Inhibitory Group that Affects Mitochondrial Uncoupling Protein 2 in Panc-1 Cells.PloS one · 2016Article
- Anti-tumor effects of various furocoumarins isolated from the roots, seeds and fruits of Angelica and Cnidium species under ultraviolet A irradiation.Journal of natural medicines · 2014Article
- A novel topoisomerase inhibitor, daurinol, suppresses growth of HCT116 cells with low hematological toxicity compared to etoposide.Neoplasia (New York, N.Y.) · 2011Article
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Authors and funding
4 authors.
Funding
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Abstract
Fluorescence pulse width can provide size information on the fluorescence-emitting particle, such as the nuclei of propidium iodide-stained cells. To analyze nuclear size in the present study, rather than perform the simple doublet discrimination approach usually employed in flow cytometric DNA content analyses, we assessed the pulse width of the propidium iodide fluorescence signal. The anti-cancer drug etoposide is reportedly cytostatic, can induce a strong G2/M arrest, and results in nuclear enlargement. Based on these characteristics, we used etoposide-treated HCT116 cells as our experimental model system. The fluorescence pulse widths (FL2-W) of etoposide-treated (10 microM, 48 h) cells were distributed at higher positions than those of vehicle control, so the peak FL2-W value of etoposide-treated cells appeared at 400 while those of vehicle control cells appeared at 200 and 270. These results were consistent with our microscopic observations. This etoposide-induced increase in FL2-W was more apparent in G2/M phase than other cell cycle phases, suggesting that etoposide-induced nuclear enlargement preferentially occurred in G2/M phase cells rather than in G0/G1 or S phase cells.
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