ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2010
The effect of phosphate accumulation on metal ion homeostasis in Saccharomyces cerevisiae.
Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 56 citations in OpenAlex.
- Approximate size and preliminary composition of the low-mass zinc pool in the cytosol of Saccharomyces cerevisiae.The Journal of biological chemistry · 2026Article
- Upper circumpolar deep water influences microbial functional gene composition and diversity along the southern Central Indian Ridge and eastern Southwest Indian Ridge.Microbiology spectrum · 2025Article
- Converging Roles of the Metal Transporter SMF11 and the Ferric Reductase FRE1 in Iron Homeostasis of Candida albicans.Molecular microbiology · 2024Article
- Phosphate availability conditions caspofungin tolerance, capsule attachment and titan cell formation inFrontiers in fungal biology · 2024Article
- The role of manganese in morphogenesis and pathogenesis of the opportunistic fungal pathogen Candida albicans.PLoS pathogens · 2023Article
- Grf10 regulates the response to copper, iron, and phosphate in Candida albicans.G3 (Bethesda, Md.) · 2023Article
- Article
- The phosphate language of fungi.Trends in microbiology · 2022Review
- Mechanisms of Acetoin Toxicity and Adaptive Responses in an Acetoin-Producing Species, Lactococcus lactis.Applied and environmental microbiology · 2021Article
- Osmolyte Signatures for the Protection ofJournal of fungi (Basel, Switzerland) · 2021Article
- Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity.Infection and immunity · 2020Article
- Identity and functions of inorganic and inositol polyphosphates in plants.The New phytologist · 2020Review
- Evolutionary Engineering of an Iron-ResistantMicroorganisms · 2019Article
- Manganese co-localizes with calcium and phosphorus inThe Journal of biological chemistry · 2019Article
- Detecting genetic interactions using parallel evolution in experimental populations.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Inorganic polyphosphates and heavy metal resistance in microorganisms.World journal of microbiology & biotechnology · 2018Review
- The biosorption of cadmium and cobalt and iron ions by yeast Cryptococcus humicola at nitrogen starvation.Folia microbiologica · 2018Article
- Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter.Nature communications · 2017Article
- Phosphate Acquisition and Virulence in Human Fungal Pathogens.Microorganisms · 2017Review
- Pho4 mediates phosphate acquisition in Candida albicans and is vital for stress resistance and metal homeostasis.Molecular biology of the cell · 2016Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
Much of what is currently understood about the cell biology of metals involves their interactions with proteins. By comparison, little is known about interactions of metals with intracellular inorganic compounds such as phosphate. Here we examined the role of phosphate in metal metabolism in vivo by genetically perturbing the phosphate content of Saccharomyces cerevisiae cells. Yeast pho80 mutants cannot sense phosphate and have lost control of phosphate uptake, storage, and metabolism. We report here that pho80 mutants specifically elevate cytosolic and nonvacuolar levels of phosphate and this in turn causes a wide range of metal homeostasis defects. Intracellular levels of the hard-metal cations sodium and calcium increase dramatically, and cells become susceptible to toxicity from the transition metals manganese, cobalt, zinc, and copper. Disruptions in phosphate control also elicit an iron starvation response, as pho80 mutants were seen to upregulate iron transport genes. The iron-responsive transcription factor Aft1p appears activated in cells with high phosphate content in spite of normal intracellular iron levels. The high phosphate content of pho80 mutants can be lowered by mutating Pho4p, the transcription factor for phosphate uptake and storage genes. Such lowering of phosphate content by pho4 mutations reversed the high calcium and sodium content of pho80 mutants and prevented the iron starvation response. However, pho4 mutations only partially reversed toxicity from heavy metals, representing a novel outcome of phosphate dysregulation. Overall, these studies underscore the importance of maintaining a charge balance in the cell; a disruption in phosphate metabolism can dramatically impact on metal homeostasis.
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