ArticleGene therapy2010
Cell-based osteoprotegerin therapy for debris-induced aseptic prosthetic loosening on a murine model.
Article in Gene therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Arthrotomy-based preclinical models of particle-induced osteolysis: A systematic review.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Pooled it
- Review
- From Development, Disease, and Decline: A Review of What Defines an Osteoclast Progenitor.International journal of molecular sciences · 2025Review
- A Murine Model of Non-Wear-Particle-Induced Aseptic Loosening.Biomimetics (Basel, Switzerland) · 2024Article
- Effect of Alumina Particles on the Osteogenic Ability of Osteoblasts.Journal of functional biomaterials · 2022Article
- Sclerostin-Mediated Impaired Osteogenesis by Fibroblast-Like Synoviocytes in the Particle-Induced Osteolysis Model.Frontiers in molecular biosciences · 2021Article
- Fibroblast-Like-Synoviocytes Mediate Secretion of Pro-Inflammatory Cytokines via ERK and JNK MAPKs in Ti-Particle-Induced Osteolysis.Materials (Basel, Switzerland) · 2020Article
- Vascular endothelial growth factor pathway promotes osseointegration and CD31The bone & joint journal · 2019Article
- Curcumin Attenuation of Wear Particle-Induced Osteolysis via RANKL Signaling Pathway Suppression in Mouse Calvarial Model.Mediators of inflammation · 2017Article
- Alteration of the RANKL/RANK/OPG System in Periprosthetic Osteolysis with Septic Loosening.Inflammation · 2016Article
- Adenoviral vector-mediated overexpression of osteoprotegerin accelerates osteointegration of titanium implants in ovariectomized rats.Gene therapy · 2015Article
- Intermittent Parathyroid Hormone Enhances Cancellous Osseointegration of a Novel Murine Tibial Implant.The Journal of bone and joint surgery. American volume · 2015Article
- Pharmacologic augmentation of implant fixation in osteopenic bone.Current osteoporosis reports · 2014Review
- Macromolecular prodrug of dexamethasone prevents particle-induced peri-implant osteolysis with reduced systemic side effects.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Periprosthetic osteolysis after total hip replacement: molecular pathology and clinical management.Inflammopharmacology · 2013Review
- Titanium particle-challenged osteoblasts promote osteoclastogenesis and osteolysis in a murine model of periprosthestic osteolysis.Acta biomaterialia · 2013Article
- Combination gene therapy targeting on interleukin-1β and RANKL for wear debris-induced aseptic loosening.Gene therapy · 2013Article
- Inhibiting periapical lesions through AAV-RNAi silencing of cathepsin K.Journal of dental research · 2013Article
- Article
- Polyethylene and metal wear particles: characteristics and biological effects.Seminars in immunopathology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Exogenous osteoprotegerin (OPG) gene modification appears a therapeutic strategy for osteolytic aseptic loosening. The feasibility and efficacy of a cell-based OPG gene delivery approach were investigated using a murine model of knee prosthesis failure. A titanium pin was implanted into mouse proximal tibia to mimic a weight-bearing knee arthroplasty, followed by titanium particles challenge to induce periprosthetic osteolysis. Mouse fibroblast-like synoviocytes were transduced in vitro with either AAV-OPG or AAV-LacZ before transfused into the osteolytic prosthetic joint 3 weeks post surgery. Successful transgene expression at the local site was confirmed 4 weeks later after killing. Biomechanical pullout test indicated a significant restoration of implant stability after the cell-based OPG gene therapy. Histology revealed that inflammatory pseudo-membranes existed ubiquitously at bone-implant interface in control groups, whereas only observed sporadically in OPG gene-modified groups. Tartrate-resistant acid phosphatase+osteoclasts and tumor necrosis factor α, interleukin-1β, CD68+ expressing cells were significantly reduced in periprosthetic tissues of OPG gene-modified mice. No transgene dissemination or tumorigenesis was detected in remote organs and tissues. Data suggest that cell-based ex vivo OPG gene therapy was comparable in efficacy with in vivo local gene transfer technique to deliver functional therapeutic OPG activities, effectively halted the debris-induced osteolysis and regained the implant stability in this model.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.