ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2010
Cholesterol-dependent kinase activity regulates transmitter release from cerebellar synapses.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Astrocytic Cholesterol Fine-Tunes the Balance of Different Modes of Synaptic Exo- and Endocytosis.bioRxiv : the preprint server for biology · 2026Article
- Gangliosides and Cholesterol: Dual Regulators of Neuronal Membrane Framework in Autism Spectrum Disorder.International journal of molecular sciences · 2025Review
- The translational potential of cholesterol-based therapies for neurological disease.Nature reviews. Neurology · 2023Review
- α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.NPJ Parkinson's disease · 2022Article
- Lipid raft-mediated and upregulated coordination pathways assist transport of glycocholic acid-modified nanoparticle in a human breast cancer cell line of SK-BR-3.International journal of pharmaceutics · 2022Article
- Mutants of theInternational journal of molecular sciences · 2021Article
- Insulin action in the brain regulates both central and peripheral functions.American journal of physiology. Endocrinology and metabolism · 2021Review
- Cholesterol modulates presynaptic and postsynaptic properties of excitatory synaptic transmission.Scientific reports · 2020Article
- Cholesterol and the Safety Factor for Neuromuscular Transmission.International journal of molecular sciences · 2019Review
- FXR-Mediated Cortical Cholesterol Accumulation Contributes to the Pathogenesis of Type A Hepatic Encephalopathy.Cellular and molecular gastroenterology and hepatology · 2018Article
- Dynasore blocks evoked release while augmenting spontaneous synaptic transmission from primary visceral afferents.PloS one · 2017Article
- Morphological Differentiation Towards Neuronal Phenotype of SH-SY5Y Neuroblastoma Cells by Estradiol, Retinoic Acid and Cholesterol.Neurochemical research · 2016Article
- Cholesterol regulates multiple forms of vesicle endocytosis at a mammalian central synapse.Journal of neurochemistry · 2015Article
- LTP-triggered cholesterol redistribution activates Cdc42 and drives AMPA receptor synaptic delivery.The Journal of cell biology · 2015Article
- Cholesterol 24S-Hydroxylase Overexpression Inhibits the Liver X Receptor (LXR) Pathway by Activating Small Guanosine Triphosphate-Binding Proteins (sGTPases) in Neuronal Cells.Molecular neurobiology · 2015Article
- Differential roles of the mevalonate pathway in the development and survival of mouse Purkinje cells in culture.Molecular neurobiology · 2015Article
- Role of membrane cholesterol in spontaneous exocytosis at frog neuromuscular synapses: reactive oxygen species-calcium interplay.The Journal of physiology · 2014Article
- Cholesterol and F-actin are required for clustering of recycling synaptic vesicle proteins in the presynaptic plasma membrane.The Journal of physiology · 2014Article
- Critical time window of neuronal cholesterol synthesis during neurite outgrowth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Changes in membrane cholesterol content can alter protein kinase activity, however, it is not known whether kinases regulating transmitter release are sensitive to membrane cholesterol content. Here we have used the cholesterol extracting agent methyl-beta-cyclodextrin to measure the effects of acute cholesterol reduction on transmitter release from cultured cerebellar neurons. Cholesterol depletion increased the frequency of spontaneous transmitter release without altering the amplitude and time course of mEPSCs. Evoked transmitter release was decreased by cholesterol extraction and the paired pulse ratio was also decreased. Alterations in synaptic transmission were not associated with failure of action potential generation or changes in presynaptic Ca(2+) signaling. Both the increase in mEPSC frequency and the change in paired pulse ratio were blocked by the broad spectrum protein kinase inhibitor staurosporine. The increase in mEPSC frequency was also sensitive to selective inhibitors of PKC and PKA. Our results therefore demonstrate that the activity of presynaptic protein kinases that regulate spontaneous and evoked neurotransmitter release is sensitive to changes of membrane cholesterol content.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.