ArticleJournal of neurophysiology2010
Calcium balance and mechanotransduction in rat cochlear hair cells.
Article in Journal of neurophysiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 108 citations in OpenAlex.
- CaProceedings of the National Academy of Sciences of the United States of America · 2026Article
- SKOOTS: Skeleton-Oriented Object Segmentation for Mitochondria in High-Resolution Cochlear EM Datasets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- SKOOTS: Skeleton-oriented object segmentation for mitochondria.bioRxiv : the preprint server for biology · 2025Article
- Hair cell apoptosis and deafness inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structural insights into calcium-dependent CIB2-TMC1 interaction in hair cell mechanotransduction.Communications biology · 2025Article
- Mechanisms driving the functional maturation of the developing mammalian auditory pathway.Current topics in developmental biology · 2025Review
- Stereocilium height changes can account for the calcium dependence of the outer-hair-cell bundle's resting state.PloS one · 2025Article
- 3D morphology of an outer-hair-cell hair bundle increases its displacement and dynamic range.Biophysical journal · 2024Article
- The pathogenic roles of the p.R130S prestin variant in DFNB61 hearing loss.The Journal of physiology · 2024Article
- Absence of oncomodulin increases susceptibility to noise-induced outer hair cell death and alters mitochondrial morphology.Frontiers in neurology · 2024Article
- In vivo investigation of mitochondria in lateral line afferent neurons and hair cells.Hearing research · 2023Review
- Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells.eLife · 2023Article
- Mitochondrial form and function in hair cells.Hearing research · 2023Review
- Structural and functional diversity of mitochondria in vestibular/cochlear hair cells and vestibular calyx afferents.Hearing research · 2022Review
- Prolonged Dexamethasone Exposure Enhances Zebrafish Lateral-Line Regeneration But Disrupts Mitochondrial Homeostasis and Hair Cell Function.Journal of the Association for Research in Otolaryngology : JARO · 2022Article
- Review
- Regulation of membrane homeostasis by TMC1 mechanoelectrical transduction channels is essential for hearing.Science advances · 2022Article
- Oncomodulin (OCM) uniquely regulates calcium signaling in neonatal cochlear outer hair cells.Cell calcium · 2022Article
- Mechanotransduction in mammalian sensory hair cells.Molecular and cellular neurosciences · 2022Review
- Synaptic Contributions to Cochlear Outer Hair Cell CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
Auditory transduction occurs by opening of Ca(2+)-permeable mechanotransducer (MT) channels in hair cell stereociliary bundles. Ca(2+) clearance from bundles was followed in rat outer hair cells (OHCs) using fast imaging of fluorescent indicators. Bundle deflection caused a rapid rise in Ca(2+) that decayed after the stimulus, with a time constant of about 50 ms. The time constant was increased by blocking Ca(2+) uptake into the subcuticular plate mitochondria or by inhibiting the hair bundle plasma membrane Ca(2+) ATPase (PMCA) pump. Such manipulations raised intracellular Ca(2+) and desensitized the MT channels. Measurement of the electrogenic PMCA pump current, which saturated at 18 pA with increasing Ca(2+) loads, indicated a maximum Ca(2+) extrusion rate of 3.7 fmol x s(-1). The amplitude of the Ca(2+) transient decreased in proportion to the Ca(2+) concentration bathing the bundle and in artificial endolymph (160 mM K(+), 20 microM Ca(2+)), Ca(2+) carried 0.2% of the MT current. Nevertheless, MT currents in endolymph displayed fast adaptation with a submillisecond time constant. In endolymph, roughly 40% of the MT current was activated at rest when using 1 mM intracellular BAPTA compared with 12% with 1 mM EGTA, which enabled estimation of the in vivo Ca(2+) load as 3 pA at rest. The results were reproduced by a model of hair bundle Ca(2+) diffusion, showing that the measured PMCA pump density could handle Ca(2+) loads incurred from resting and maximal MT currents in endolymph. The model also indicated the endogenous mobile buffer was equivalent to 1 mM BAPTA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.