ArticleMolecular biology of the cell2010
Positive charges of translocating polypeptide chain retrieve an upstream marginal hydrophobic segment from the endoplasmic reticulum lumen to the translocon.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Insulin gene mutations and posttranslational and translocation defects: associations with diabetes.Endocrine · 2020Pooled it
- Role of Pbr1, a putative oxidoreductase, in the ER quality control and folding of yeast Fks1 glucan synthase.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The V5-Epitope Tag for Cell Engineering and Its Use in Immunohistochemistry and Quantitative Flow Cytometry.Biology · 2025Article
- ATP2, The essential P4-ATPase of malaria parasites, catalyzes lipid-stimulated ATP hydrolysis in complex with a Cdc50 β-subunit.Emerging microbes & infections · 2021Article
- Interaction mapping of the Sec61 translocon identifies two Sec61α regions interacting with hydrophobic segments in translocating chains.The Journal of biological chemistry · 2018Article
- Positive charge in the n-region of the signal peptide contributes to efficient post-translational translocation of small secretory preproteins.The Journal of biological chemistry · 2018Article
- Stability and flexibility of marginally hydrophobic-segment stalling at the endoplasmic reticulum translocon.Molecular biology of the cell · 2016Article
- Review
- INS-gene mutations: from genetics and beta cell biology to clinical disease.Molecular aspects of medicine · 2015Review
- Mechanisms of integral membrane protein insertion and folding.Journal of molecular biology · 2015Review
- Inefficient translocation of preproinsulin contributes to pancreatic β cell failure and late-onset diabetes.The Journal of biological chemistry · 2014Article
- Orientational preferences of neighboring helices can drive ER insertion of a marginally hydrophobic transmembrane helix.Molecular cell · 2012Article
- Membrane protein insertion at the endoplasmic reticulum.Annual review of cell and developmental biology · 2011Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Positively charged amino acid residues are well recognized topology determinants of membrane proteins. They contribute to the stop-translocation of a polypeptide translocating through the translocon and to determine the orientation of signal sequences penetrating the membrane. Here we analyzed the function of these positively charged residues during stop-translocation in vitro. Surprisingly, the positive charges facilitated membrane spanning of a marginally hydrophobic segment, even when separated from the hydrophobic segment by 70 residues. In this case, the hydrophobic segment was exposed to the lumen, and then the downstream positive charges triggered the segment to slide back into the membrane. The marginally hydrophobic segment spanned the membrane, but maintained access to the water environment. The positive charges not only fix the hydrophobic segment in the membrane at its flanking position, but also have a much more dynamic action than previously realized.
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