ArticleThe Journal of clinical endocrinology and metabolism2010
Vitamin D-binding protein directs monocyte responses to 25-hydroxy- and 1,25-dihydroxyvitamin D.
Article in The Journal of clinical endocrinology and metabolism, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 5 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
109 citing papers in PubMed, 5 syntheses or guidelines pooled it, 226 citations in OpenAlex.
- Perspective: Vitamin D supplementation prevents rickets and acute respiratory infections when given as daily maintenance but not as intermittent bolus: implications for COVID-19.Clinical medicine (London, England) · 2021Pooled it
- Which Risk Factors Causally Influence Dementia? A Systematic Review of Mendelian Randomization Studies.Journal of Alzheimer's disease : JAD · 2018Pooled it
- Association of vitamin D status with arterial blood pressure and hypertension risk: a mendelian randomisation study.The lancet. Diabetes & endocrinology · 2014Pooled it
- Causal relationship between obesity and vitamin D status: bi-directional Mendelian randomization analysis of multiple cohorts.PLoS medicine · 2013Pooled it
- The nonskeletal effects of vitamin D: an Endocrine Society scientific statement.Endocrine reviews · 2012Pooled it
- Trial
- Effects of vitamin D supplementation on circulating concentrations of growth factors and immune-mediators in healthy women during pregnancy.Pediatric research · 2021Trial
- Inflammation Modulation by Vitamin D and Calcium in the Morphologically Normal Colorectal Mucosa of Patients with Colorectal Adenoma in a Clinical Trial.Cancer prevention research (Philadelphia, Pa.) · 2021Trial
- Associations Between Change in Total and Free 25-Hydroxyvitamin D With 24,25-Dihydroxyvitamin D and Parathyroid Hormone.The Journal of clinical endocrinology and metabolism · 2018Trial
- Effects of High-Dose Vitamin D2 Versus D3 on Total and Free 25-Hydroxyvitamin D and Markers of Calcium Balance.The Journal of clinical endocrinology and metabolism · 2016Trial
- Vitamin D-binding protein levels do not influence the effect of vitamin D repletion on serum PTH and calcium: data from a randomized, controlled trial.The Journal of clinical endocrinology and metabolism · 2014Trial
- Changes in vitamin D-related mineral metabolism after induction with anti-tumor necrosis factor-α therapy in Crohn's disease.The Journal of clinical endocrinology and metabolism · 2014Trial
- Vitamin D Enhancement of Adipose Biology: Implications on Obesity-Associated Cardiometabolic Diseases.Nutrients · 2025Review
- Potential pathogenic and protective genotypes and phenotypes of vitamin D binding protein in multiple sclerosis.Frontiers in neurology · 2025Article
- Relation of T Cell Profile with Vitamin D Receptor and Vitamin D-Binding Protein Gene Polymorphisms in Atopy.International journal of molecular sciences · 2024Article
- Vitamin D Deficiency, Chronic Kidney Disease and Periodontitis.Medicina (Kaunas, Lithuania) · 2024Review
- Seasonal variation of total and bioavailable 25-hydroxyvitamin D [25(OH)D] in the healthy adult Slovenian population.Acta biochimica Polonica · 2024Article
- Vitamin D receptor and vitamin D binding protein gene polymorphisms in patients with asthma: a pilot study.BMC pulmonary medicine · 2023Article
- Vitamin D and COVID-19: where are we now?Postgraduate medicine · 2023Review
- Article
49 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 4 countries.
Funding
Abstract
backgroundSerum 25-hydroxyvitamin D (25OHD) is a key factor in determining monocyte induction of the antimicrobial protein cathelicidin, which requires intracrine conversion of 25OHD to 1,25-dihydroxyvitamin D [1,25(OH)(2)D]. Both vitamin D metabolites circulate bound to vitamin D-binding protein (DBP), but the effect of this on induction of monocyte cathelicidin remains unclear.
methodsHuman monocytes were cultured in medium containing 1) serum from DBP knockout (DBP(-/-)) or DBP(+/-) mice, 2) serum-free defined supplement reconstituted with DBP or albumin (control), and 3) human serum with different DBP [group-specific component [Gc]] genotypes with varying affinities for vitamin D metabolites. In each case, response to added 1,25(OH)(2)D(3) or 25OHD(3) was determined by measuring expression of mRNA for cathelicidin and 24-hydroxylase. Monocyte internalization of DBP was assessed by fluorescent tagging followed by microscopic and flow cytometric analysis of tagged DBP.
resultsMonocytes cultured in DBP(-/-) serum showed more potent induction of cathelicidin by 25OHD(3) or 1,25(OH)(2)D(3) when compared with DBP(+/-) serum. Likewise, DBP added to serum-free medium attenuated 25OHD(3)/1,25(OH)(2)D(3) responses. Fluorescently tagged DBP showed low-level uptake by monocytes, but this did not appear to involve a megalin-mediated mechanism. Human serum containing low-affinity Gc2-1S or Gc2-2, respectively, supported 2.75-fold (P = 0.003) and 2.43-fold (P = 0.016) higher induction of cathelicidin by 25OHD relative to cells cultured with high affinity Gc1F-1F.
conclusionThese data indicate that DBP plays a pivotal role in regulating the bioavailablity of 25OHD to monocytes. Vitamin D-dependent antimicrobial responses are therefore likely to be strongly influenced by DBP polymorphisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.