ArticleNucleic acids research2010
Combination of RNA interference and U1 inhibition leads to increased inhibition of gene expression.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Minor intron splicing is critical for survival of lethal prostate cancer.Molecular cell · 2023Article
- U1A is a positive regulator of the expression of heterologous and cellular genes involved in cell proliferation and migration.Molecular therapy. Nucleic acids · 2022Article
- ISR8/IRF1-AS1 Is Relevant for IFNα and NF-κB Responses.Frontiers in immunology · 2022Article
- Ankyrin G Membrane Partners Drive the Establishment and Maintenance of the Axon Initial Segment.Frontiers in cellular neuroscience · 2017Article
- Complementary Effects of Interleukin-15 and Alpha Interferon Induce Immunity in Hepatitis B Virus Transgenic Mice.Journal of virology · 2016Article
- Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication.EMBO reports · 2016Article
- Long Non-Coding RNA BST2/BISPR is Induced by IFN and Regulates the Expression of the Antiviral Factor Tetherin.Frontiers in immunology · 2014Article
- Increased in vivo inhibition of gene expression by combining RNA interference and U1 inhibition.Nucleic acids research · 2012Article
- The dose can make the poison: lessons learned from adverse in vivo toxicities caused by RNAi overexpression.Silence · 2011Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) has been revolutionary for the specific inhibition of gene expression. However, the application of RNAi has been hampered by the fact that many siRNAs induce dose-dependent unwanted secondary effects. Therefore, new methods to increase inhibition of gene expression with low doses of inhibitors are required. We have tested the combination of RNAi and U1i (U1 small nuclear RNA--snRNA--interference). U1i is based on U1 inhibitors (U1in), U1 snRNA molecules modified to target a pre-mRNA and inhibit its gene expression by blocking nuclear polyadenylation. The combination of RNAi and U1i resulted in stronger inhibition of reporter or endogenous genes than that obtained using either of the techniques alone. The increased inhibition observed is stable over time and allows higher inhibition than the best obtained with either of the inhibitors alone even with decreased doses of the inhibitors. We believe that the combination of RNAi and U1i will be of interest when higher inhibition is required or when potent inhibitors are not available. Also, the combination of these techniques would allow functional inhibition with a decreased dose of inhibitors, avoiding toxicity due to dose-dependent unwanted effects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.