ArticleThe Journal of biological chemistry2010
Peroxynitrite toxicity in Escherichia coli K12 elicits expression of oxidative stress responses and protein nitration and nitrosylation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- A rapid prototyped atmospheric non-thermal plasma-activated aerosol device and anti-bacterial characterisation.Frontiers in chemistry · 2024Article
- Nitrites in Cured Meats, Health Risk Issues, Alternatives to Nitrites: A Review.Foods (Basel, Switzerland) · 2022Review
- Nitric oxide precipitates catastrophic chromosome fragmentation by bolstering both hydrogen peroxide and Fe(II) Fenton reactants in E. coli.The Journal of biological chemistry · 2022Article
- Tyrosine Nitration of Flagellins: a Response of Sinorhizobium meliloti to Nitrosative Stress.Applied and environmental microbiology · 2020Article
- Erwinia amylovora catalases KatA and KatG are virulence factors and delay the starvation-induced viable but non-culturable (VBNC) response.Molecular plant pathology · 2018Article
- Structural characterization of the bacterial proteasome homolog BPH reveals a tetradecameric double-ring complex with unique inner cavity properties.The Journal of biological chemistry · 2018Article
- Adaptation ofGenetics · 2017Article
- Computational Analysis of Host-Pathogen Protein Interactions between Humans and Different Strains of EnterohemorrhagicFrontiers in cellular and infection microbiology · 2017Article
- N-acetyl ornithine deacetylase is a moonlighting protein and is involved in the adaptation of Entamoeba histolytica to nitrosative stress.Scientific reports · 2016Article
- Carbon Monoxide Gas Is Not Inert, but Global, in Its Consequences for Bacterial Gene Expression, Iron Acquisition, and Antibiotic Resistance.Antioxidants & redox signaling · 2016Article
- Response to Gaseous NO2 Air Pollutant of P. fluorescens Airborne Strain MFAF76a and Clinical Strain MFN1032.Frontiers in microbiology · 2016Article
- Article
- Oral streptococci and nitrite-mediated interference of Pseudomonas aeruginosa.Infection and immunity · 2015Article
- Article
- Transcriptomic and phenotypic analysis of paralogous spx gene function in Bacillus anthracis Sterne.MicrobiologyOpen · 2013Article
- Redox proteomics uncovers peroxynitrite-sensitive proteins that help Escherichia coli to overcome nitrosative stress.The Journal of biological chemistry · 2013Article
- S-nitrosylation regulates mitochondrial quality control via activation of parkin.Scientific reports · 2013Article
- Stressor-induced increase in microbicidal activity of splenic macrophages is dependent upon peroxynitrite production.Infection and immunity · 2012Article
- Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations.Open biology · 2012Article
- The intestinal microbiota are necessary for stressor-induced enhancement of splenic macrophage microbicidal activity.Brain, behavior, and immunity · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Peroxynitrite is formed in macrophages by the diffusion-limited reaction of superoxide and nitric oxide. This highly reactive species is thought to contribute to bacterial killing by interaction with diverse targets and nitration of protein tyrosines. This work presents for the first time a comprehensive analysis of transcriptional responses to peroxynitrite under tightly controlled chemostat growth conditions. Up-regulation of the cysteine biosynthesis pathway and an increase in S-nitrosothiol levels suggest S-nitrosylation to be a consequence of peroxynitrite exposure. Genes involved in the assembly/repair of iron-sulfur clusters also show enhanced transcription. Unexpectedly, arginine biosynthesis gene transcription levels were also elevated after treatment with peroxynitrite. Analysis of the negative regulator for these genes, ArgR, showed that post-translational nitration of tyrosine residues within this protein is responsible for its degradation in vitro. Further up-regulation was seen in oxidative stress response genes, including katG and ahpCF. However, genes known to be up-regulated by nitric oxide and nitrosating agents (e.g. hmp and norVW) were unaffected. Probabilistic modeling of the transcriptomic data identified five altered transcription factors in response to peroxynitrite exposure, including OxyR and ArgR. Hydrogen peroxide can be present as a contaminant in commercially available peroxynitrite preparations. Transcriptomic analysis of cells treated with hydrogen peroxide alone also revealed up-regulation of oxidative stress response genes but not of many other genes that are up-regulated by peroxynitrite. Thus, the cellular responses to peroxynitrite and hydrogen peroxide are distinct.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.