ArticleBioorganic & medicinal chemistry2010
A collection of caged compounds for probing roles of local translation in neurobiology.
Article in Bioorganic & medicinal chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Opto-chemogenetic inhibition of L-type CaCell reports methods · 2024Article
- Spatiotemporally resolved protein synthesis as a molecular framework for memory consolidation.Trends in neurosciences · 2022Review
- Targeted protein oxidation using a chromophore-modified rapamycin analog.Chemical science · 2021Article
- Chemical and Light Inducible Epigenome Editing.International journal of molecular sciences · 2020Review
- A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation Initiation.ACS synthetic biology · 2019Article
- Photoactivatable Caged Prodrugs of VEGFR-2 Kinase Inhibitors.Molecules (Basel, Switzerland) · 2016Article
- Light control of cellular processes by using photocaged abscisic acid.Chembiochem : a European journal of chemical biology · 2015Article
- Light-controlled synthetic gene circuits.Current opinion in chemical biology · 2012Review
- Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP.Journal of the American Chemical Society · 2011Article
- A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity.Journal of the American Chemical Society · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Spatially localized translation plays a vital role in the normal functioning of neuronal systems and is widely believed to be involved in both learning and memory formation. It is of central interest to understand both the phenomenon and molecular mechanisms of local translation using new tools and approaches. Caged compounds can, in principle, be used as tools to investigate local translation since optical activation of bioactive molecules can achieve both spatial and temporal resolution on the micron scale and on the order of seconds or less, respectively. Successful caging of bioactive molecules requires the identification of key functional groups in appropriate molecules and the introduction of a suitable caging moiety. Here we present the design, synthesis and testing of a collection of three caged compounds: anisomycin caged with a diethylaminocoumarin moiety and dimethoxynitrobenzyl caged versions of 4E-BP and rapamycin. Whereas caged anisomycin can be used to control general translation, caged 4E-BP serves as a probe of cap-dependent translation initiation and caged rapamycin serves a probe of the role of mTORC1 in translation initiation. In vitro translation assays demonstrate that these caging strategies, in combination with the aforementioned compounds, are effective for optical control making it likely that such strategies can successfully employed in the study of local translation in living systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.