ArticleBMC plant biology2010
Large impact of the apoplast on somatic embryogenesis in Cyclamen persicum offers possibilities for improved developmental control in vitro.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- The glutathione S-transferase gene LaGSTU6 promotes somatic embryogenesis in a recalcitrant lily genotype by regulating scales redox homeostasis.Molecular horticulture · 2026Article
- Secretome analysis revealed that cell wall remodeling and starch catabolism underlie the early stages of somatic embryogenesis inFrontiers in plant science · 2023Article
- Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern.Frontiers in plant science · 2022Article
- Russeting in 'Apple' Mango: Triggers and Mechanisms.Plants (Basel, Switzerland) · 2020Article
- Embryogenic Callus as Target for Efficient Transformation ofFrontiers in plant science · 2018Article
- Article
- De novo assembly and comparative analysis of the transcriptome of embryogenic callus formation in bread wheat (Triticum aestivum L.).BMC plant biology · 2017Article
- Differential proteome analysis during early somatic embryogenesis in Musa spp. AAA cv. Grand Naine.Plant cell reports · 2017Article
- Metabolite profiling of somatic embryos of Cyclamen persicum in comparison to zygotic embryos, endosperm, and testa.Frontiers in plant science · 2015Article
- Transcriptome Profiling of Wheat Seedlings following Treatment with Ultrahigh Diluted Arsenic Trioxide.Evidence-based complementary and alternative medicine : eCAM · 2014Article
- From callus to embryo: a proteomic view on the development and maturation of somatic embryos in Cyclamen persicum.Planta · 2012Article
- Enolases: storage compounds in seeds? Evidence from a proteomic comparison of zygotic and somatic embryos of Cyclamen persicum Mill.Plant molecular biology · 2011Article
- Developmental localization and methylesterification of pectin epitopes during somatic embryogenesis of banana (Musa spp. AAA).PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClonal propagation is highly desired especially for valuable horticultural crops. The method with the potentially highest multiplication rate is regeneration via somatic embryogenesis. However, this mode of propagation is often hampered by the occurrence of developmental aberrations and non-embryogenic callus. Therefore, the developmental process of somatic embryogenesis was analysed in the ornamental crop Cyclamen persicum by expression profiling, comparing different developmental stages of embryogenic cell cultures, zygotic vs. somatic embryos and embryogenic vs. non-embryogenic cell cultures.
resultsThe analysis was based on a cDNA microarray representing 1,216 transcripts and was exemplarily validated by realtime PCR. For this purpose relative transcript abundances of homologues of a putative receptor kinase, two different glutathione S-transferases (GST), a xyloglucan endotransglycosylase (XET) and a peroxidase (POX) were quantitatively measured by realtime PCR for three different comparisons. In total, 417 genes were found to be differentially expressed. Gene Ontology annotation revealed that transcripts coding for enzymes that are active in the extracellular compartment (apoplast) were significantly overrepresented in several comparisons. The expression profiling results are underpinned by thorough histological analyses of somatic and zygotic embryos.
conclusionsThe putative underlying physiological processes are discussed and hypotheses on improvement of the protocol for in vitro somatic embryogenesis in Cyclamen persicum are deduced. A set of physiological markers is proposed for efficient molecular control of the process of somatic embryogenesis in C. persicum. The general suitability of expression profiling for the development and improvement of micropropagation methods is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.