ArticleThe Journal of biological chemistry2010
Forward genetic analysis reveals multiple gating mechanisms of TRPV4.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- The effect of type-2-diabetes-related vascular endothelial dysfunction on skin physiology and activities of daily living.Journal of diabetes science and technology · 2011Trial
- Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery.Bioscience reports · 2024Article
- Subcellular mechano-regulation of cell migration in confined extracellular microenvironment.Biophysics reviews · 2023Review
- De novoJournal of medical genetics · 2022Article
- Positive modulation of the TMEM16B mediated currents by TRPV4 antagonist.Biochemistry and biophysics reports · 2021Article
- TRPing to the Point of Clarity: Understanding the Function of the Complex TRPV4 Ion Channel.Cells · 2021Review
- TRPing on Cell Swelling - TRPV4 Senses It.Frontiers in immunology · 2021Review
- Cell sensing and decision-making in confinement: The role of TRPM7 in a tug of war between hydraulic pressure and cross-sectional area.Science advances · 2019Article
- Human mutations highlight an intersubunit cation-π bond that stabilizes the closed but not open or inactivated states of TRPV channels.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Structures of TRPV2 in distinct conformations provide insight into role of the pore turret.Nature structural & molecular biology · 2019Article
- Review
- TRPV4 (Transient Receptor Potential Vanilloid 4) Channel-Dependent Negative Feedback Mechanism Regulates GArteriosclerosis, thrombosis, and vascular biology · 2018Article
- When size matters: transient receptor potential vanilloid 4 channel as a volume-sensor rather than an osmo-sensor.The Journal of physiology · 2017Article
- A competing hydrophobic tug on L596 to the membrane core unlatches S4-S5 linker elbow from TRP helix and allows TRPV4 channel to open.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Gain-of-function mutation in TRPV4 identified in patients with osteonecrosis of the femoral head.Journal of medical genetics · 2016Article
- Function and regulation of TRPP2 ion channel revealed by a gain-of-function mutant.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A channelopathy mechanism revealed by direct calmodulin activation of TrpV4.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Sensitisation of TRPV4 by PAR2 is independent of intracellular calcium signalling and can be mediated by the biased agonist neutrophil elastase.Pflugers Archiv : European journal of physiology · 2015Article
- L596-W733 bond between the start of the S4-S5 linker and the TRP box stabilizes the closed state of TRPV4 channel.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Yeast luminometric and Xenopus oocyte electrophysiological examinations of the molecular mechanosensitivity of TRPV4.Journal of visualized experiments : JoVE · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
TRPV4 is a polymodal cation channel gain-of-function (GOF) allele which causes skeletal dysplasia in humans. To better understand its gating, we screened for additional GOF alleles based on their ability to block yeast proliferation. Repeatedly, only a limited number of such growth-blocking mutations were isolated. Expressed in oocytes, wild-type channels can be strongly activated by either hypotonicity or exposure to the potent agonist 4alphaPDD, although the GOF channels behaved as if they were fully prestimulated as well as lacking a previously uncharacterized voltage-dependent inactivation. Five of six mutations occurred at or near the inner ends of the predicted core helices, giving further direct evidence that this region indeed forms the main intracellular gate in TRP channels. Surprisingly, both wild-type channels as well as these GOF channels maintain strong steady-state outward rectification that is not due to a Ca(2+) block, as has been proposed elsewhere. We conclude that TRPV4 contains an additional voltage-dependent gating mechanism in series with the main intracellular gate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.