ArticlePlant physiology2010
An intron sense suppression construct targeting soybean FAD2-1 requires a double-stranded RNA-producing inverted repeat T-DNA insert.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Multiplex FAD2/FATB Editing Generates Ultra-High-Oleic, Low-Saturate Soybean With Increased Seed Fatty Acid Content.Plant biotechnology journal · 2026Article
- Genomic and biochemical comparison of allelic triple-mutant lines derived from conventional breeding and multiplex gene editing.The plant genome · 2025Article
- KeyInternational journal of molecular sciences · 2023Article
- Carbon Dots for Efficient Small Interfering RNA Delivery and Gene Silencing in Plants.Plant physiology · 2020Article
- Association Study of the 5'UTR Intron of theFrontiers in plant science · 2020Article
- Improved oil quality in transgenic soybean seeds by RNAi-mediated knockdown of GmFAD2-1B.Transgenic research · 2018Article
- Development of high oleic oil crop platform in flax through RNAi-mediated multiple FAD2 gene silencing.Plant cell reports · 2015Article
- Replicating Potato spindle tuber viroid mediates de novo methylation of an intronic viroid sequence but no cleavage of the corresponding pre-mRNA.RNA biology · 2015Article
- Effect of orientation of transcription of a gene in an inverted transferred DNA repeat on transcriptional gene silencing in rice transgenics-a case study.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- A large and functionally diverse family of Fad2 genes in safflower (Carthamus tinctorius L.).BMC plant biology · 2013Article
- Posttranscriptional gene silencing in nuclei.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate that the transformation of soybean (Glycine max) with sense suppression constructs using intron sequences from the fatty acid oleyl Delta12 desaturase gene FAD2-1A leads to efficient and specific reduction of FAD2-1 transcripts in developing seeds, increased oleic acid, and decreased polyunsaturated fatty acids. The related FAD2-2 transcripts are only marginally affected. Despite screening a large number of independent transformants, no single-copy efficacious transformants could be found. Invariably, all the least complex transgenic loci have two T-DNA copies in an inverted repeat configuration, centered at the right borders. We show that this T-DNA configuration produces an inverted repeat transcript and that small interfering RNAs accumulate against the target sequence.
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