ArticleEpigenetics & chromatin2010
HP1gamma function is required for male germ cell survival and spermatogenesis.
Article in Epigenetics & chromatin, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Review
- HP1-Mediated Silencing of theJournal of developmental biology · 2025Article
- The Role of Polycomb Proteins in Cell Lineage Commitment and Embryonic Development.Epigenomes · 2022Review
- ADAD2 regulates heterochromatin in meiotic and post-meiotic male germ cells via translation of MDC1.Journal of cell science · 2022Article
- Heterochromatin protein 1 beta regulates neural and neural crest development by repressing pluripotency-associated gene pou5f3.2/oct25 in Xenopus.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- The roles of Polycomb repressive complexes in mammalian development and cancer.Nature reviews. Molecular cell biology · 2021Review
- HP1γ regulates H3K36 methylation and pluripotency in embryonic stem cells.Nucleic acids research · 2020Article
- How HP1 Post-Translational Modifications Regulate Heterochromatin Formation and Maintenance.Cells · 2020Review
- Review
- A genomics approach to females with infertility and recurrent pregnancy loss.Human genetics · 2020Article
- On the relations of phase separation and Hi-C maps to epigenetics.Royal Society open science · 2020Article
- Heterochromatin protein 1 (HP1) is intrinsically required for post-transcriptional regulation of Drosophila Germline Stem Cell (GSC) maintenance.Scientific reports · 2019Article
- Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines.Disease markers · 2019Article
- Mechanisms Underlying the Regulation of HP1γ by the NGF-PKA Signaling Pathway.Scientific reports · 2018Article
- Deletion of HP1γ in cardiac myocytes affects H4K20me3 levels but does not impact cardiac growth.Epigenetics & chromatin · 2018Article
- Compartmentalization of HP1 Proteins in Pluripotency Acquisition and Maintenance.Stem cell reports · 2018Article
- Comparative Transcriptome Analysis Provides Insights into Differentially Expressed Genes and Long Non-Coding RNAs between Ovary and Testis of the Mud Crab (Scylla paramamosain).Marine biotechnology (New York, N.Y.) · 2018Article
- Single-molecule kinetic analysis of HP1-chromatin binding reveals a dynamic network of histone modification and DNA interactions.Nucleic acids research · 2017Article
- An HP1 isoform-specific feedback mechanism regulates Suv39h1 activity under stress conditions.Epigenetics · 2017Article
- Heterochromatin and the molecular mechanisms of 'parent-of-origin' effects in animals.Journal of biosciences · 2016Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHP1 proteins are conserved components of eukaryotic constitutive heterochromatin. In mammals, there are three genes that encode HP1-like proteins, termed HP1alpha, HP1beta and HP1gamma, which have a high degree of homology This paper describes for the first time, to our knowledge, the physiological function of HP1gamma using a gene-targeted mouse.
resultsWhile targeting the Cbx3 gene (encoding the HP1gamma protein) with a conditional targeting vector, we generated a hypomorphic allele (Cbx3hypo), which resulted in much reduced (barely detectable) levels of HP1gamma protein. Homozygotes for the hypomorphic allele (Cbx3hypo/hypo) are rare, with only 1% of Cbx3hypo/hypo animals reaching adulthood. Adult males exhibit a severe hypogonadism that is associated with a loss of germ cells, with some seminiferous tubules retaining only the supporting Sertoli cells (Sertoli cell-only phenotype). The percentage of seminiferous tubules that are positive for L1 ORF1 protein (ORF1p) in Cbx3hypo/hypo testes is greater than that for wild-type testes, indicating that L1 retrotransposon silencing is reversed, leading to ectopic expression of ORF1p in Cbx3hypo/hypo germ cells.
conclusionsThe Cbx3 gene product (the HP1gamma protein) has a non-redundant function during spermatogenesis that cannot be compensated for by the other two HP1 isotypes. The Cbx3hypo/hypo spermatogenesis defect is similar to that found in Miwi2 and Dnmt3L mutants. The Cbx3 gene-targeted mice generated in this study provide an appropriate model for the study of HP1gamma in transposon silencing and parental imprinting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.