ArticlePLoS genetics2010
Fragilities caused by dosage imbalance in regulation of the budding yeast cell cycle.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- The Dynamic Fungal Genome: Polyploidy, Aneuploidy and Copy Number Variation in Response to Stress.Annual review of microbiology · 2023Review
- Article
- Functional diversification accompanies gene family expansion of MED2 homologs in Candida albicans.PLoS genetics · 2018Article
- Cellular Stress Associated with Aneuploidy.Developmental cell · 2018Review
- Post-Translational Dosage Compensation Buffers Genetic Perturbations to Stoichiometry of Protein Complexes.PLoS genetics · 2017Article
- Quantitative Systems Biology to decipher design principles of a dynamic cell cycle network: the "Maximum Allowable mammalian Trade-Off-Weight" (MAmTOW).NPJ systems biology and applications · 2017Review
- Network analyses based on comprehensive molecular interaction maps reveal robust control structures in yeast stress response pathways.NPJ systems biology and applications · 2016Article
- Experimental detection of short regulatory motifs in eukaryotic proteins: tips for good practice as well as for bad.Cell communication and signaling : CCS · 2015Review
- Quantitative nature of overexpression experiments.Molecular biology of the cell · 2015Article
- Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW.BMC systems biology · 2014Article
- Control analysis of the eukaryotic cell cycle using gene copy-number series in yeast tetraploids.BMC genomics · 2013Article
- Predicting complex phenotype-genotype interactions to enable yeast engineering: Saccharomyces cerevisiae as a model organism and a cell factory.Biotechnology journal · 2013Review
- Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method.Genome research · 2013Article
- Review
- Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.PloS one · 2013Article
- The genetic control of growth rate: a systems biology study in yeast.BMC systems biology · 2012Article
- Overexpression limits of fission yeast cell-cycle regulators in vivo and in silico.Molecular systems biology · 2011Article
- The aneuploidy paradox: costs and benefits of an incorrect karyotype.Trends in genetics : TIG · 2011Review
- Dosage suppression genetic interaction networks enhance functional wiring diagrams of the cell.Nature biotechnology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells can maintain their functions despite fluctuations in intracellular parameters, such as protein activities and gene expression levels. This commonly observed biological property of cells is called robustness. On the other hand, these parameters have different limitations, each reflecting the property of the subsystem containing the parameter. The budding yeast cell cycle is quite fragile upon overexpression of CDC14, but is robust upon overexpression of ESP1. The gene products of both CDC14 and ESP1 are regulated by 1ratio1 binding with their inhibitors (Net1 and Pds1), and a mathematical model predicts the extreme fragility of the cell cycle upon overexpression of CDC14 and ESP1 caused by dosage imbalance between these genes. However, it has not been experimentally shown that dosage imbalance causes fragility of the cell cycle. In this study, we measured the quantitative genetic interactions of these genes by performing combinatorial "genetic tug-of-war" experiments. We first showed experimental evidence that dosage imbalance between CDC14 and NET1 causes fragility. We also showed that fragility arising from dosage imbalance between ESP1 and PDS1 is masked by CDH1 and CLB2. The masking function of CLB2 was stabilization of Pds1 by its phosphorylation. We finally modified Chen's model according to our findings. We thus propose that dosage imbalance causes fragility in biological systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.