ArticlePloS one2010
Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- The Role of Sperm Proteins IZUMO1 and TMEM95 in Mammalian Fertilization: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Decoding the Genes Orchestrating Egg and Sperm Fusion Reactions and Their Roles in Fertility.Biomedicines · 2024Review
- Mapping the proteome landscape of Indian Zebu (Sahiwal) spermatozoa using high-resolution mass spectrometry andAnimal biotechnology · 2024Article
- C11orf94/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly.Science advances · 2022Article
- Angiotensin-converting enzyme 2 (ACE2): Two decades of revelations and re-evaluation.Peptides · 2022Review
- Characterization of the Testis-Specific Angiotensin Converting Enzyme (tACE)-Interactome during Bovine Sperm Capacitation.Current issues in molecular biology · 2022Article
- COVID-19 and male reproductive system: pathogenic features and possible mechanisms.Journal of molecular histology · 2021Review
- Variants in ACE2; potential influences on virus infection and COVID-19 severity.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2021Review
- Regulation of Male Fertility by the Renin-Angiotensin System.International journal of molecular sciences · 2020Review
- The molecular basis of fertilization (Review).International journal of molecular medicine · 2016Review
- Angiotensin-Converting Enzyme 3 (ACE3) Protects Against Pressure Overload-Induced Cardiac Hypertrophy.Journal of the American Heart Association · 2016Article
- The Immunoglobulin-like Gene spe-45 Acts during Fertilization in Caenorhabditis elegans like the Mouse Izumo1 Gene.Current biology : CB · 2015Article
- Molecular and cellular mechanisms of sperm-oocyte interactions opinions relative to in vitro fertilization (IVF).International journal of molecular sciences · 2014Review
- Sperm-egg fusion: a molecular enigma of mammalian reproduction.International journal of molecular sciences · 2014Review
- Unresolved questions concerning mammalian sperm acrosomal exocytosis.Biology of reproduction · 2014Review
- Mechanism of fertilization: a modern view.Experimental animals · 2014Review
- ACE2 - from the renin-angiotensin system to gut microbiota and malnutrition.Microbes and infection · 2013Review
- Paternal fenvalerate exposure influences reproductive functions in the offspring.Reproductive sciences (Thousand Oaks, Calif.) · 2013Article
- Angiotensin-converting enzymes play a dominant role in fertility.International journal of molecular sciences · 2013Review
- Critical role of exosomes in sperm-egg fusion and virus-induced cell-cell fusion.Reproductive medicine and biology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIZUMO1 is the only sperm protein which is proven to be essential for sperm-egg fusion. However, the IZUMO1 is a structurally simple protein with single Ig domain and seems not to include either a "fusogenic peptide" or a fusion machinery domain. This led us to assume the existence of an IZUMO1-interacting protein(s) which makes a functional fusion machine interacting with IZUMO1. METHODOLOGY/PRINCIPAL
findingsWe produced a transgenic mouse line which expresses His-tagged IZUMO1 in the Izumo1(-/-) genetic background. After solubilization of sperm membranes, we purified His-tagged IZUMO1 using anti-His affinity chromatography and found a protein that interacts with IZUMO1. After being separated on SDS-PAGE gel, the IZUMO1-interacting protein was subjected to LC-MS/MS analysis and from the partial fragments, we identified the protein as ACE3. We raised the antibody against ACE3 and found that ACE3 is localized on the acrosomal cap area as in the case of IZUMO1. However, ACE3 disappeared from sperm after acrosome reaction while IZUMO1 remained on sperm. In order to investigate the role of ACE3 in vivo, we generated Ace3-deficient mice by homologous recombination and examined the fertilizing ability of the males. Unexpectedly, the male mice showed no defect in fertilizing ability in in vivo or in an in vitro fertilization system. CONCLUSIONS/SIGNIFICANCE: We identified an IZUMO1-interacting protein in sperm, which we identified as testis specific ACE homologue ACE3. We produced an Ace3 disrupted mouse line, and found the localization of IZUMO1 spread in a little wider area on sperm, but the elimination of ACE3 did not result in a loss of sperm fertilizing ability, differing from the case of ACE disruption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.