ArticleJournal of lipid research2010
Extracellular cholesterol-rich microdomains generated by human macrophages and their potential function in reverse cholesterol transport.
Article in Journal of lipid research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Snapshots of ABCG1 and ABCG5/G8: A Sterol's Journey to Cross the Cellular Membranes.International journal of molecular sciences · 2022Review
- Different Pathways of Cellular Cholesterol Efflux.Cell biochemistry and biophysics · 2022Review
- Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Macrophage-Derived Cholesterol Contributes to Therapeutic Resistance in Prostate Cancer.Cancer research · 2021Article
- A new pathway of macrophage cholesterol efflux.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cultured macrophages transfer surplus cholesterol into adjacent cells in the absence of serum or high-density lipoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Ultramorphological analysis of plaque advancement and cholesterol crystal formation in Ldlr knockout mouse atherosclerosis.Atherosclerosis · 2019Article
- Why Is Very High Cholesterol Content Beneficial for the Eye Lens but Negative for Other Organs?Nutrients · 2019Review
- Macrophages release plasma membrane-derived particles rich in accessible cholesterol.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Two polymorphic cholesterol monohydrate crystal structures form in macrophage culture models of atherosclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Macrophages Shed Excess Cholesterol in Unique Extracellular Structures Containing Cholesterol Microdomains.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- ABCA1 (ATP-Binding Cassette Transporter A1) Mediates ApoA-I (Apolipoprotein A-I) and ApoA-I Mimetic Peptide Mobilization of Extracellular Cholesterol Microdomains Deposited by Macrophages.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Development of Correlative Cryo-soft X-ray Tomography and Stochastic Reconstruction Microscopy. A Study of Cholesterol Crystal Early Formation in Cells.Journal of the American Chemical Society · 2016Article
- Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages.Journal of visualized experiments : JoVE · 2016Article
- High-density lipoprotein-mediated transcellular cholesterol transport in mouse aortic endothelial cells.Biochemical and biophysical research communications · 2015Article
- ABCA1 contributes to macrophage deposition of extracellular cholesterol.Journal of lipid research · 2015Article
- Macrophages, extracellular matrix, and lipoproteins in arterial cholesterol balance.Journal of lipid research · 2014Article
- ABCG1-mediated generation of extracellular cholesterol microdomains.Journal of lipid research · 2014Article
- Spontaneous formation of two-dimensional and three-dimensional cholesterol crystals in single hydrated lipid bilayers.Biophysical journal · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Previous studies have shown that cholesterol in atherosclerotic plaques is present in both intracellular and extracellular forms. In the current study, we investigated a mechanism for extracellular cholesterol accumulation and examined the capacity of this pool of cholesterol to be removed by cholesterol acceptors, a step in reverse cholesterol transport. Human monocyte-derived macrophages differentiated with macrophage-colony stimulating factor were incubated with acetylated LDL to allow cholesterol enrichment and processing. These macrophages were subsequently labeled with a monoclonal antibody that specifically detects ordered cholesterol arrays, revealing the presence of unesterified cholesterol-rich microdomains on the cell surfaces and in the extracellular matrix. Similar unesterified cholesterol-rich microdomains were present in human atherosclerotic plaques. Actin microfilaments functioned in microdomain deposition or maintenance, and Src family kinases regulated transfer of these microdomains from the cell surface onto the extracellular matrix. Mediators of reverse cholesterol transport, apolipoprotein A-I (apoA-I), and HDL were capable of removing these extracellular un-esterified cholesterol-rich microdomains. However, apoA-I removed the microdomains only when macrophages were present. ApoA-I removal of microdomains was blocked by glyburide and inhibitor of ATP-binding cassette transporter A1 (ABCA1) function. In summary, cultures of cholesterol-enriched human monocyte-derived macrophages generate extracellular unesterified cholesterol-rich microdomains, which can subsequently be removed by cholesterol acceptors and therefore potentially function in reverse cholesterol transport.
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