ArticleThe Journal of cell biology2010
LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Apical PAR-3 caps orient the mitotic spindle inbioRxiv : the preprint server for biology · 2023Article
- Article
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Article
- The polarity-induced force imbalance inMolecular biology of the cell · 2018Article
- Microtubule Feedback and LET-99-Dependent Control of Pulling Forces Ensure Robust Spindle Position.Biophysical journal · 2018Article
- The kinases PIG-1 and PAR-1 act in redundant pathways to regulate asymmetric division in the EMS blastomere of C. elegans.Developmental biology · 2018Article
- Article
- Tumor suppressor APC is an attenuator of spindle-pulling forces duringProceedings of the National Academy of Sciences of the United States of America · 2018Article
- LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring.Molecular biology of the cell · 2017Article
- Increased lateral microtubule contact at the cell cortex is sufficient to drive mammalian spindle elongation.Molecular biology of the cell · 2017Article
- Stronger net posterior cortical forces and asymmetric microtubule arrays produce simultaneous centration and rotation of the pronuclear complex in the early Caenorhabditis elegans embryo.Molecular biology of the cell · 2016Article
- Mitotic Spindle Positioning in the EMS Cell of Caenorhabditis elegans Requires LET-99 and LIN-5/NuMA.Genetics · 2016Article
- Centrosome centering and decentering by microtubule network rearrangement.Molecular biology of the cell · 2016Article
- Engineered non-Mendelian inheritance of entire parental genomes in C. elegans.Nature biotechnology · 2016Article
- The 14-3-3 protein PAR-5 regulates the asymmetric localization of the LET-99 spindle positioning protein.Developmental biology · 2016Article
- Pericentriolar material structure and dynamics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- The biology of the germ line in echinoderms.Molecular reproduction and development · 2014Review
- DEPDC1/LET-99 participates in an evolutionarily conserved pathway for anti-tubulin drug-induced apoptosis.Nature cell biology · 2014Article
- Mechanisms of spindle positioning.The Journal of cell biology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cortical pulling on astral microtubules positions the mitotic spindle in response to PAR polarity cues and G protein signaling in many systems. In Caenorhabditis elegans single-cell embryos, posterior spindle displacement depends on Galpha and its regulators GPR-1/2 and LIN-5. GPR-1/2 and LIN-5 are necessary for cortical pulling forces and become enriched at the posterior cortex, which suggests that higher forces act on the posterior spindle pole compared with the anterior pole. However, the precise distribution of cortical forces and how they are regulated remains to be determined. Using spindle severing, single centrosome assays, and centrosome fragmentation, we show that both the anterior and posterior cortices generate more pulling force than the lateral-posterior region. Lateral inhibition depends on LET-99, which inhibits GPR-1/2 localization to produce a bipolar GPR-1/2 pattern. Thus, rather than two domains of cortical force, there are three. We propose that the attenuation of lateral forces prevents counterproductive pulling, resulting in a higher net force toward the posterior that contributes to spindle elongation and displacement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.