ArticleInfection and immunity2010
NlpI contributes to Escherichia coli K1 strain RS218 interaction with human brain microvascular endothelial cells.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- STEC in the natural environment of Uruguay: genomic surveillance and environmental circulation in the framework of One Health.Microbiology spectrum · 2026Article
- Low leucine levels in the blood enhance the pathogenicity of neonatal meningitis-causing Escherichia coli.Nature communications · 2025Article
- Structural basis for recruitment of peptidoglycan endopeptidase MepS by lipoprotein NlpI.Nature communications · 2024Article
- Construction and application of the conditionally essential gene knockdown library inApplied and environmental microbiology · 2023Article
- The Diversity ofMicroorganisms · 2023Review
- Genetic components ofFrontiers in microbiology · 2023Article
- Biomimetic Lipopolysaccharide-Free Bacterial Outer Membrane-Functionalized Nanoparticles for Brain-Targeted Drug Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- cjrABC-senB hinders survival of extraintestinal pathogenic E. coli in the bloodstream through triggering complement-mediated killing.Journal of biomedical science · 2020Article
- Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi-enzyme complexes in Escherichia coli.The EMBO journal · 2020Article
- The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli.Journal of biomedical science · 2020Article
- Identification of MltG as a Prc Protease Substrate Whose Dysregulation Contributes to the Conditional Growth Defect of Prc-DeficientFrontiers in microbiology · 2020Article
- Peptidoglycan Endopeptidase Spr of UropathogenicFrontiers in microbiology · 2020Article
- Antibiotic resistance and molecular characterization of bacteremia Escherichia coli isolates from newborns in the United States.PloS one · 2019Article
- Factors Involved in the Persistence of a Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Some Gram-negative Lipoproteins Keep Their Surface Topology When Transplanted from One Species to Another and Deliver Foreign Polypeptides to the Bacterial Surface.Molecular & cellular proteomics : MCP · 2017Article
- A journey into the brain: insight into how bacterial pathogens cross blood-brain barriers.Nature reviews. Microbiology · 2017Review
- Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier.PLoS pathogens · 2016Article
- Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier.Oncotarget · 2016Article
- Human Meningitis-Associated Escherichia coli.EcoSal Plus · 2016Review
- Characterizing the pathotype of neonatal meningitis causing Escherichia coli (NMEC).BMC microbiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Escherichia coli K1 is the most common Gram-negative bacillary organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMECs) is a prerequisite for its traversal of the blood-brain barrier (BBB) and penetration into the brain. In the present study, we identified NlpI as a novel bacterial determinant contributing to E. coli K1 interaction with HBMECs. The deletion of nlpI did not affect the expression of the known bacterial determinants involved in E. coli K1-HBMEC interaction, such as type 1 fimbriae, flagella, and OmpA, and the contribution of NlpI to HBMECs binding and invasion was independent of those bacterial determinants. Previous reports have shown that the nlpI mutant of E. coli K-12 exhibits growth defect at 42 degrees C at low osmolarity, and its thermosensitive phenotype can be suppressed by a mutation on the spr gene. The nlpI mutant of strain RS218 exhibited similar thermosensitive phenotype, but additional spr mutation did not restore the ability of the nlpI mutant to interact with HBMECs. These findings suggest the decreased ability of the nlpI mutant to interact with HBMECs is not associated with the thermosensitive phenotype. NlpI was determined as an outer membrane-anchored protein in E. coli, and the nlpI mutant was defective in cytosolic phospholipase A(2)alpha (cPLA(2)alpha) phosphorylation compared to the parent strain. These findings illustrate the first demonstration of NlpI's contribution to E. coli K1 binding to and invasion of HBMECs, and its contribution is likely to involve cPLA(2)alpha.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.