ArticleNucleic acids research2010
Drosophila melanogaster Dis3 N-terminal domains are required for ribonuclease activities, nuclear localization and exosome interactions.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- DIS3L2 knockdown impairs key oncogenic properties of colorectal cancer cells via the mTOR signaling pathway.Cellular and molecular life sciences : CMLS · 2023Article
- Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function.The Journal of biological chemistry · 2020Article
- RNA Exosomes and Their Cofactors.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Comprehensive analysis of nucleocytoplasmic dynamics of mRNA in Drosophila cells.PLoS genetics · 2017Article
- Characterization of a TUTase/RNase complex required forRNA (New York, N.Y.) · 2017Article
- Collaborative Control of Cell Cycle Progression by the RNA Exonuclease Dis3 and Ras Is Conserved Across Species.Genetics · 2016Article
- The 3' to 5' Exoribonuclease DIS3: From Structure and Mechanisms to Biological Functions and Role in Human Disease.Biomolecules · 2015Review
- The 3'-5' exoribonuclease Dis3 regulates the expression of specific microRNAs in Drosophila wing imaginal discs.RNA biology · 2015Article
- Proteins involved in the degradation of cytoplasmic mRNA in the major eukaryotic model systems.RNA biology · 2014Review
- Threading the barrel of the RNA exosome.Trends in biochemical sciences · 2013Review
- The mitochondrial RNA landscape of Saccharomyces cerevisiae.PloS one · 2013Article
- Dis3- and exosome subunit-responsive 3' mRNA instability elements.Biochemical and biophysical research communications · 2012Article
- Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.Yeast (Chichester, England) · 2011Article
- The exozyme model: a continuum of functionally distinct complexes.RNA (New York, N.Y.) · 2011Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic cells use numerous pathways to regulate RNA production, localization and stability. Several of these pathways are controlled by ribonucleases. The essential ribonuclease, Dis3, plays important roles in distinct RNA metabolic pathways. Despite much progress in understanding general characteristics of the Dis3 enzyme in vitro and in vivo, much less is known about the contributions of Dis3 domains to its activities, subcellular localization and protein-protein interactions. To address these gaps, we constructed a set of Drosophila melanogaster Dis3 (dDis3) mutants and assessed their enzymatic activity in vitro and their localizations and interactions in S2 tissue culture cells. We show that the dDis3 N-terminus is sufficient for endoribonuclease activity in vitro and that proper N-terminal domain structure is critical for activity of the full-length polypeptide. We find that the dDis3 N-terminus also contributes to its subcellular distribution, and is necessary and sufficient for interactions with core exosome proteins. Finally, dDis3 interaction with dRrp6 and dImportin-α3 is independent of core interactions and occurs though two different regions. Taken together, our data suggest that the dDis3 N-terminus is a dynamic and complex hub for RNA metabolism and exosome interactions.
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Registered trials
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