ArticleJournal of experimental botany2010
Two solanesyl diphosphate synthases with different subcellular localizations and their respective physiological roles in Oryza sativa.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 39 citations in OpenAlex.
- Mysterious giants in the world of lipids: long linear isoprenoid functions in plant physiology and reproduction.Journal of experimental botany · 2026Review
- Structural insights into the molecular mechanisms of OsFBN5-induced OsSPS3 catalysis.Nature plants · 2026Article
- Systematic characterization and functional analysis ofFrontiers in plant science · 2025Article
- The Inferior Grain Filling Initiation Promotes the Source Strength of Rice Leaves.Rice (New York, N.Y.) · 2023Article
- How plants synthesize coenzyme Q.Plant communications · 2022Review
- A review of starch biosynthesis in cereal crops and its potential breeding applications in rice (PeerJ · 2021Article
- The Predicted Functional Compartmentation of Rice Terpenoid Metabolism byInternational journal of molecular sciences · 2020Article
- Effects ofPlants (Basel, Switzerland) · 2020Article
- Current Status and Future Prospects in Herbicide Discovery.Plants (Basel, Switzerland) · 2019Review
- Functional Analysis of Polyprenyl Diphosphate Synthase Genes Involved in Plastoquinone and Ubiquinone Biosynthesis inFrontiers in plant science · 2019Article
- Solanesol Biosynthesis in Plants.Molecules (Basel, Switzerland) · 2017Review
- Conserved Function of Fibrillin5 in the Plastoquinone-9 Biosynthetic Pathway in Arabidopsis and Rice.Frontiers in plant science · 2017Article
- Organ- and Growing Stage-Specific Expression of Solanesol Biosynthesis Genes in Nicotiana tabacum Reveals Their Association with Solanesol Content.Molecules (Basel, Switzerland) · 2016Article
- Plastoquinone and Ubiquinone in Plants: Biosynthesis, Physiological Function and Metabolic Engineering.Frontiers in plant science · 2016Review
- Fibrillin 5 Is Essential for Plastoquinone-9 Biosynthesis by Binding to Solanesyl Diphosphate Synthases in Arabidopsis.The Plant cell · 2015Article
- Functional modeling identifies paralogous solanesyl-diphosphate synthases that assemble the side chain of plastoquinone-9 in plastids.The Journal of biological chemistry · 2013Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Long chain prenyl diphosphates are crucial biosynthetic precursors of ubiquinone (UQ) in many organisms, ranging from bacteria to humans, as well as precursors of plastoquinone in photosynthetic organisms. The cloning and characterization of two solanesyl diphosphate synthase genes, OsSPS1 and OsSPS2, in Oryza sativa is reported here. OsSPS1 was highly expressed in root tissue whereas OsSPS2 was found to be high in both leaves and roots. Enzymatic characterization using recombinant proteins showed that both OsSPS1 and OsSPS2 could produce solanesyl diphosphates as their final product, while OsSPS1 showed stronger activity than OsSPS2. However, an important biological difference was observed between the two genes: OsSPS1 complemented the yeast coq1 disruptant, which does not form UQ, whereas OsSPS2 only very weakly complemented the growth defect of the coq1 mutant. HPLC analyses showed that both OsSPS1 and OsSPS2 yeast transformants produced UQ9 instead of UQ6, which is the native yeast UQ. According to the complementation study, the UQ9 levels in OsSPS2 transformants were much lower than that of OsSPS1. Green fluorescent protein fusion analyses showed that OsSPS1 localized to mitochondria, while OsSPS2 localized to plastids. This suggests that OsSPS1 is involved in the supply of solanesyl diphosphate for ubiquinone-9 biosynthesis in mitochondria, whereas OsSPS2 is involved in providing solanesyl diphosphate for plastoquinone-9 formation. These findings indicate that O. sativa has a different mechanism for the supply of isoprenoid precursors in UQ biosynthesis from Arabidopsis thaliana, in which SPS1 provides a prenyl moiety for UQ9 at the endoplasmic reticulum.
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