ArticleMethods in molecular biology (Clifton, N.J.)2010
High-quality immunofluorescence of cultured cells.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
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- Examining Phosphatases Through Immunofluorescent Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- IST1 regulates select recycling pathways.Traffic (Copenhagen, Denmark) · 2024Article
- Zebrafish Patient-Derived Xenograft Model as a Preclinical Platform for Uveal Melanoma Drug Discovery.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence.International journal of molecular sciences · 2022Review
- MON2 Guides Wntless Transport to the Golgi through Recycling Endosomes.Cell structure and function · 2020Article
- ER arrival sites associate with ER exit sites to create bidirectional transport portals.The Journal of cell biology · 2020Article
- Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks.Journal of visualized experiments : JoVE · 2018Article
- Pseudopodium-enriched atypical kinase 1 mediates angiogenesis by modulating GATA2-dependent VEGFR2 transcription.Cell discovery · 2018Article
- No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells.PloS one · 2017Article
- A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays.Histochemistry and cell biology · 2016Article
- Restoration of R117H CFTR folding and function in human airway cells through combination treatment with VX-809 and VX-770.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Cell and Tissue Display: An Alternative Multipurpose Tool for Microscopy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2016Article
- Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study.Journal of visualized experiments : JoVE · 2016Article
- A faster immunofluorescence assay for tracking infection progress of human cytomegalovirus.Acta biochimica et biophysica Sinica · 2012Article
- Sec12 binds to Sec16 at transitional ER sites.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Immunofluorescence microscopy of cultured cells often gives poor preservation of delicate structures. We have obtained dramatically improved results with a simple modification of a standard protocol. Cells growing on a coverslip are rapidly dehydrated in a cold organic solvent and then are rehydrated in a solution containing a homobifunctional crosslinker. The crosslinking reaction stabilizes cellular structures during subsequent incubation and wash steps, usually without compromising antigenicity. This method reproducibly yields high-quality images of endomembrane compartments and cytoskeletal elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.