Evidence map›Paper›PMID 20419411›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2010

Characterization of interactions between proteins using site-directed spin labeling and electron paramagnetic resonance spectroscopy.

Jennine M Crane, Angela A Lilly, Linda L Randall

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
5.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Direct visualization reveals dynamics of a transient intermediate during protein assembly.Proceedings of the National Academy of Sciences of the United States of America · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jennine M CraneDepartment of Biochemistry, University of Missouri, Columbia, MO, USA.
Angela A Lilly
Linda L Randall
University of Missouri · US

Funding

The Export of Proteins in Escherichia Coli; Administrative Supplement for EquipmentR01GM029798 · NIGMS · WASHINGTON STATE UNIVERSITY · PI RANDALL, LINDA L. · 1985 to 2019
$7.7M
NIGMS NIH HHS R01 GM029798
6 · The paper itself

Abstract

Site-directed spin-labeling and the analysis of proteins by electron paramagnetic resonance spectroscopy provides a powerful tool for identifying sites of contact within protein complexes at the resolution of aminoacyl side chains. Here we describe the method as we have used it to study interactions of proteins involved in export via the Sec secretory system in Escherichia coli. The method is amendable to the study of most protein interactions.

Indexed as

Spin LabelsElectron Spin Resonance SpectroscopyProtein BindingProteinsProteinsSpin Labels

Identifiers

PMID20419411
PMCPMC5814136
OpenAlexW2411984669

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.